Avenanthramide and Saponin Bioavailability in Oat Bran
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 13
- 主要终点
- Bioavailability of Avenanthramides and Saponins in Urine
研究概览
简要总结
Avenanthramides and saponins are types of chemical compounds found naturally in oats.
Avenanthramides have anti-oxidant properties, anti-atherosclerotic, anti-inflammation, and anti-proliferative effects on cancer cells in vitro. Oat saponins, or avenacosides, have the ability to bind cholesterol, and thus, the ability to lower blood cholesterol.
Oat bran is a known source of these dietary compounds. This study aims to determine the bioavailability of these compounds to in the urine of participants after ingesting an oat bran cereal, both before, and after for multiple time points.
Analytical chemistry will be used to determine the bioavailability of the oat compounds at each time point. This will help to establish a kinetic curve for the metabolism of these compounds.
详细描述
Initially, the diet of the participants will be asked to restricted to avoid eating any polyphenols that might interfere with the employed analytical chemistry techniques. This period of diet restriction is the washout period. The list of foods to avoid are: oats, whole grains, fruits, vegetables, herbal supplements, ginger, coffee, tea, and chocolate.
After the washout period, a baseline urine and fecal sample will be collected from the participants, then a large portion of oat bran will be provided to the participants for consumption. The approximate portion size will be 100 g by dry weight. The participants will then provide urine samples during the designated time points. The time points for the urine collection are as follows:
- st sample, ~ 30 min to 1 hour prior to oat consumption
- nd sample: 0-2 hours after oat consumption
- rd sample: 2-4 hours after oat consumption
- th sample: 4-6 hours after oat consumption
- th sample: 6-9 hours after oat consumption
- th sample: 9-12 hours after oat consumption
- th sample: 12-24 hours after oat consumption
- th sample: 24-32 hours after oat consumption
- th sample: 48 hours after oat consumption These samples will be analyzed in using HPLC and LC/MS.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 50 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •BMI 18.5-25
- •Have blood and urine biochemical markers in normal range
- •Have no known allergy to oat relate foods
- •Be not taking antibiotics for six months
- •Be not currently taking medication
- •Be nonsmoking
- •Have no alcoholic intoxication
- •Have no extensive exposure to industrial waste
排除标准
- •Individuals with gout
- •Individuals with heart disease
- •Individuals with peripheral vascular diseases
- •Individuals with degenerative liver
- •Cancer patients
- •Patients with diabetes
- •Individuals with GI disorders
- •Individuals with endocrine disorders
结局指标
主要结局
Bioavailability of Avenanthramides and Saponins in Urine
时间窗: 32-48 hours post-dose
Liquid chromatography-tandem mass spectrometry will be utilized to assess the bioavailability of avenanthramides and saponins in urine.
次要结局
未报告次要终点
研究者
Shengmin Sang
Principal Investigator
North Carolina Agriculture & Technical State University