Investigation of the Endothel Dependent and Independent Vasodilatation in Human Gingiva: Comparison of the Effect of NO and ACH
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 24
- 试验地点
- 1
- 主要终点
- Blood flow change after applying the test solutions
研究概览
简要总结
The aim of this study is to compare the endothelim-dependent and non-dependent vasodilatation between genders in the human gingiva.
详细描述
Gender differences were observed in studies according to gingival microcirculation between physiological circumstances and during wound healing as well.
For blood flow measurement Laser Spackle Contrast Imaging is a reliable tool. It has good reproducibility, it doesn't get in touch with the measured surface, it's easy to use and it can measure on a bigger surface in same time.
Two wells were fabricated on the labial surface of the right lateral incisor (test tooth) and on the labial surface of the left central incisor (control tooth). These wells are opened towards the high-prermeability gingival sulcus, so the dropped solutions nitroglycerine vs physiological saline or nitroglycerine vs physiological saline can esasily penetrate to the gingival tissues. After applying the solutions, the blood flow was monitored fot 15 minutes.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 20 Years 至 30 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •general health
- •minimum 5 mm keratinized gingiva at the upper front teeth
- •good oral hygiene
排除标准
- •pregnancy and breast-feeding
- •medication
- •insufficient marginal integrity
- •periodontitis
研究组 & 干预措施
nitroglycerin
NO donor (Nitropohl) vs pgysiological saline
1mg/ml nitorhlycerin
干预措施: Nitroglycerin (Drug)
acethylcholine
10 mg/ml acetylcholine vs physiological saline
干预措施: Acetylcholine (Drug)
结局指标
主要结局
Blood flow change after applying the test solutions
时间窗: 20 minutes
The change in blood flow will be measeured after applying the test solutions.
次要结局
- Comparison the change in blood flow between the two solutions(30 minutes)
- Comparison the change in blood flow in genders(20 minutes)
研究者
Dr. Vag Janos
associate professor
Semmelweis University