Lipid and Carbohydrate Metabolism: Effects of Exercise
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Insulin Resistance
- Sponsor
- Yale University
- Enrollment
- 41
- Locations
- 1
- Primary Endpoint
- change in de novo lipogenesis
- Status
- Completed
- Last Updated
- last year
Overview
Brief Summary
The purpose of this study was to examine the pathophysiology of insulin resistance and the relation to the metabolic syndrome in humans.
Detailed Description
In this study insulin resistant (IR), young offspring of parents with type 2 diabetes (18-46 years) and healthy, lean elderly individuals (65-90 years) underwent 1H/13C MRS measurements of liver and muscle lipid and glycogen content before and after a high carbohydrate meal challenge. Hepatic de novo lipogenesis was determined as the incorporation of deuterium, from deuterium labeled water (2H2O), into plasma triglycerides 5. These metabolic studies were performed in 2 sets: Study Part 1: Resting, Study Part 2: After 1 bout of 45 minutes exercise. The study has been completed and published as two papers: August 16, 2011 and July 24, 2012.
Investigators
Eligibility Criteria
Inclusion Criteria
- •Body Mass Index \< 29 kg/m2
- •No regular exercise routine
- •Non-smokers
- •Absence of systemic and organ disease.
Exclusion Criteria
- •Hematocrit \< 35 vol%
- •History of claustrophobia
- •Ferromagnetic implants
- •Regular exercise regimen
- •Not weight stable
- •Any significant hematological, neurological, musculoskeletal or endocrine disease (including diabetes mellitus)
- •Any significant cardiovascular condition, including confirmed CAD and prior cardiovascular events
- •Taking coumadin as anticoagulant therapy.
Outcomes
Primary Outcomes
change in de novo lipogenesis
Time Frame: Baseline
At the baseline assessment, de novo lipogenesis was assessed after a high carbohydrate meal and a drink of heavy water. Muscle glycogen and lipid contents were measured pre- and post meal using 1H/13C MRS and de novo lipogenesis was assessed from the heavy water incorporation into blood lipid in samples collected overnight.