Cellular and Molecular Inflammatory Mechanisms Leading to Bone Resorption in Peri-implantitis Vs Periodontitis

Completed

• Conditions: Peri-Implantitis; Periodontitis
• Interventions: Procedure: Open Flap Debridement; Procedure: Crown lengthening

• Registration Number: NCT02664675
• Lead Sponsor: University of Paris 5 - Rene Descartes

Brief Summary

Recent data found that peri-implantitis may differ from periodontitis at the histological level in terms of extent and composition of the cellular components. From human biopsies, it has been shown a deeper apical extension of the inflammatory cell infiltrate and a larger proportion of granulocytes and macrophages in peri-implantitis lesions compared to periodontitis lesions. Following experimental peri-implantitis, it has been observed a continuing bone loss around dental implants after ligature removal; whereas, such a progression was not observed around teeth after cessation of the experimental periodontitis. The factors influencing the recruitment and the activation of osteoclasts in both diseases have not been compared yet.

Hypothesis : The more rapid and more severe alveolar bone destruction occurring in peri-implantitis compared to periodontitis is due at least in part to differences in the inflammatory process in these diseases.

Aim: to study the cytokine profile produced by gingival explants from periodontitis or peri-implantitis sites and to test the consequences on alteration of bone remodelling activity using in vitro approach.

Materials and methods The investigators will establish a collection of human gingival samples from patients undergoing surgical treatment of periodontitis or peri-implantitis. The investigators will test pro-inflammatory cytokines and chemokines release and consequences on osteoclast differentiation and activity.

Detailed Description

Not available

Study Timeline

• Study Start: 2014-04
• Study First Submitted: 2014-04-25
• Primary Completion: 2015-04
• Status Verified: 2016-01
• Study Completion: 2016-01
• Study First Submitted QC: 2016-01-22
• Study First Posted: 2016-01-27
• Last Update Submitted: 2016-01-27
• Last Update Posted: 2016-01-28

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 33

Inclusion Criteria

• Beneficiary by national health insurance

• Age: over 18 year-old

• Non smoking

• Good health

  ---For the periimplantitis group:

• patients formerly treated with a non surgical procedure without antibiotics with at least one functional dental implant with at least on pocket deeper than 5 mm with bleeding on probing and radiographical alveolar bone loss.

  ---For the periodontitis group:

• patients formerly treated with a non surgical procedure without antibiotics for a generalized severe chronic periodontitis (in accord to Armitage 2009) and needing a resective surgical procedure (periodontal pockets deeper than 5 mm with bleeding on probing)

  ---For the healthy group:

• patients with good health without periodontal or peri implant disease needing gingivectomy (crown lengthening, esthetic surgery)

Exclusion Criteria

• Smokers
• Patients treated with antibiotics or NSAID during the last 3 months
• Patients suffering from a chronic infectious, inflammatory or auto-immune disease (non equilibrated diabetes mellitus, rheumatoid arthritis, spondylarthrosis, Gougerot-Sjögren, Crohn, Behcet, Horton, obesity, chronic end renal disease, cardiopathies....)
• Pregnant women
• For the healthy group : bleeding on probing on the site.

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Periimplantitis	Open Flap Debridement	patients formerly treated with a non surgical procedure without antibiotics with at least one functional dental implant with at least on pocket deeper than 5 mm with bleeding on probing and radiographical alveolar bone loss. These patient will be treated by open flap debridement nd the granulation tissue xill be harvested for analysis.
Periodontitis	Open Flap Debridement	patients formerly treated with a non surgical procedure without antibiotics for a generalized severe chronic periodontitis (in accord to Armitage 2009) and needing a resective surgical procedure (periodontal pockets deeper than 5 mm with bleeding on probing) These patient will be treated by open flap debridement nd the granulation tissue xill be harvested for analysis.
Healthy patient	Crown lengthening	healthy patients needing crown lengthening allowing collection of gingival explants

Primary Outcome Measures

Name	Time	Method
Interleukine 17 (IL-17) releasing from periodontitis and peri-implantitis gingival samples	24 hours of incubation	Interleukine 17 (IL-17) releasing in the medium from harvested granulation tissue from periodontitis, peri-implantitis and healthy patients will be determine by ELISA test 24 hours after the beginning of the culture.

Trial Locations

Locations (2)

Hospital Rotschild; 🇫🇷 Paris, France

Hospital Charles Foix APHP; 🇫🇷 Ivry Sur Seine, France