A Phase II, Randomized, Two Stage, Double-Blind, Placebo-Controlled, Safety and Immunogenicity Study of an Intramuscular Influenza Vaccine (Multimeric-001) Injected to Healthy Volunteers
Overview
- Phase
- Phase 2
- Intervention
- Not specified
- Conditions
- Influenza
- Sponsor
- BiondVax Pharmaceuticals ltd.
- Enrollment
- 200
- Locations
- 2
- Primary Endpoint
- Number of Participants with Adverse Events as a Measure of Safety and Tolerability
- Status
- Completed
- Last Updated
- 13 years ago
Overview
Brief Summary
This is a multi-center, randomized, two stage, double-blind, placebo-controlled administration study comprising 200 healthy participants.
Detailed Description
This is a Phase II multi-center, randomized, two stage, double-blind, placebo-controlled study comprising 200 participants. Eligible subjects will be randomized to receive one of the following administrations (as two single IM injections with an interval of 21 days between each injection Administration A: Prime twice with Adjuvanted Multimeric-001 500 mcg - 64 subjects. Administration B: PBS (Placebo) twice - 32 subjects. Administration C: Adjuvanted PBS (Placebo)twice - 32 subjects. Participants from administrations A and B will be further immunized with a 15% dose of commercial seasonal trivalent vaccine for 2011 on day 81. Administration D: Adjuvanted Multimeric-001 500 mcg coadministered once with 15% of TIV dose - 24 subjects. Administration E: Adjuvanted Multimeric-001 500 mcg coadministered once with 50% of TIV dose - 24 subjects Administration group F: PBS (Placebo) co-administered once with 50% of TIV dose - 24 subjects
Investigators
Eligibility Criteria
Inclusion Criteria
- •Healthy males and females between the age of 18 and 49 years (inclusive).
- •Subjects who provide written informed consent to participate in the study.
- •Subjects able to adhere to the visit schedule and protocol requirements and be available to complete the study.
- •Haematology, chemistry and urinalysis values with no clinical significance or do not reflect a medical condition which, according to the physician's judgment, might confound the results of the study or pose additional risk to the subject by participation in the study.
- •Female of childbearing age must agree to use an acceptable method of contraception and male subjects should use a condom throughout the study period (until termination visit) if female partner is not using an effective contraceptive method.
- •Subjects who are seronegative to at least one of the strains included in the seasonal vaccine against influenza for 2011.
Exclusion Criteria
- •Known history of significant medical disorder, which in the investigator's judgment, might confound the results of the study or pose additional risk to the subject by participation in the study.
- •Subjects with known Guillain Barré Syndrome in the past.
- •Two or more hospitalizations within the last year prior to screening visit.
- •Known bleeding disorders including hemophilia or thrombocytopenia, or treatment with anticoagulant therapy (risk of bleeding with intramuscular injection).
- •Immunocompromised patients and those receiving concomitant immunosuppressive therapy; or other immune modulating drugs including chronic steroid treatment.
- •Subjects who have been immunized with anti-influenza vaccine or infected by influenza virus within eight months prior to the screening visit.
- •Administration of any vaccine 30 days before the screening visit.
- •Known hypersensitivity to previous influenza vaccination.
- •Use of an influenza antiviral medication within 4 weeks of vaccination.
- •Known hypersensitivity and/or allergy to any drug or vaccine.
Outcomes
Primary Outcomes
Number of Participants with Adverse Events as a Measure of Safety and Tolerability
Time Frame: From day 0 until day 221
Number of Participants with Adverse Events possible/probably related to the study drug in each group were similar in the experimental and control groups.
Anti Multimeric-001 antibodies
Time Frame: 21 days after second immunization with M-001
Direct Elisa assay to test the titer of human antibodies that recognize the Multimeric-001 protein. Humoral immunity was manifested 21 and 60 days post immunization by significantly elevated anti-M-001 IgG levels among subjects administered twice with adjuvanted M-001
Secondary Outcomes
- Hemagglutination Inhibition (HAI) test for anti influenza antibodies(21 days post co administration of M-001 and TIV 50%)