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Single-Dose Intravenous Inositol Pharmacokinetics in Preterm Infants

Phase 2
Completed
Conditions
Infant, Low Birth Weight
Retinopathy of Prematurity
Bronchopulmonary Dysplasia (BPD)
Infant, Small for Gestational Age
Infant, Premature
Infant, Newborn
Interventions
Drug: Placebo higher volume
Drug: Placebo lower volume
Registration Number
NCT00349726
Lead Sponsor
NICHD Neonatal Research Network
Brief Summary

This pilot study was a randomized, placebo-controlled, clinical trial to measure changes in blood and urine levels of inositol in premature infants at high risk for retinopathy of prematurity (ROP) following a single intravenous dose of inositol. Based on previous studies, the premise is that maintaining inositol concentrations similar to those occurring naturally in utero will reduce the rates of ROP and bronchopulmonary dysplasia in premature infants. The objective was to evaluate the single-dose pharmacokinetics and safety of different amounts of intravenous myo-inositol (provided by Ross Products Division, Abbott Laboratories) in very low birth weight neonates, in preparation for a future Phase III multi-center randomized controlled trial. This study enrolled 74 infants at high risk for retinopathy at 9 NICHD Neonatal Research Network sites, and randomly assigned them to receive either 60mg/kg of 5% inositol, 120 mg/kg of 5% inositol, 60 mg/kg of 5% glucose (the placebo), or 120 mg/kg of 5% glucose.

Detailed Description

Retinopathy of prematurity (ROP) is an abnormal growth of the blood vessels in the eye that occurs primarily in very premature infants. Eye development occurs normally in the womb; in infants born prematurely, however, the blood vessels must finish developing outside the protective environment of the uterus. Retinopathy of prematurity (also known as retrolental fibroplasia) is a leading cause of blindness and other vision impairments (myopia, strabismus, and amblyopia) in children, both in developed and developing countries.

Inositol is a naturally-occurring sugar alcohol produced by the placenta and is present in high levels in fetal blood throughout pregnancy in humans and other animals. Serum levels fall rapidly after birth, although this fall is moderated in infants who receive breast milk. Two randomized trials have shown that intravenous inositol supplementation in the first week significantly reduced death, bronchopulmonary dysplasia (BPD), and retinopathy. One study of oral supplements was less convincing, but also supported reduction of retinopathy.

This pilot study evaluated the half-life pharmacokinetics of a single-dose of myo-inositol (provided by Ross Products Division, Abbott Laboratories) in very low birth weight infants, looking at changes in blood and urine inositol levels. The premise is that maintaining inositol concentrations similar to those occurring naturally in utero will reduce the rates of retinopathy and bronchopulmonary dysplasia in premature infants. Results from this study will be used to select the doses for a subsequent multi-dose pilot study, and for the planned large multi-center trials.

In this study, nine NICHD Neonatal Research Network sites enrolled 74 infants of less than 30 weeks gestation and randomly assigned them to receive either 60mg/kg of 5% inositol, 120 mg/kg of 5% inositol, 60 mg/kg of 5% glucose (the placebo), or 120 mg/kg of 5% glucose. Concentrations of inositol were measured in both blood and urine to determine population pharmacokinetic parameters for these infants.

Stratification: Enrolled infants were stratified by age with 37 infants of 23 0/7 to 26 6/7 weeks in one group and 37 infants of 27 0/7 to 29 6/7 weeks in a second group.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
74
Inclusion Criteria
  • 23 0/7 to 26 6/7 weeks gestational age (36 infants) or
  • 27 0/7 to 29 6/7 weeks gestational age (36 infants)
  • 600-1500 grams birth weight
  • No enteral feedings since birth at enrollment
  • 3-6 days (25-132 hours) postnatal age

Note: Because of the high mortality expected in this population (15-20%), the study design (originally for 72 infants) required recruitment of a replacement subject if any infant failed to complete the four blood samples during the first week of the study.

Exclusion Criteria
  • Major congenital anomalies
  • Moribund or not to be provided continued support
  • Renal failure suspected (creatinine >2.5 with oliguria)
  • Exchange transfusion received or expected to receive

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Inositol high volumeInositol higher volumeSingle dose of intravenous inositol 5%, 120 mg/kg (2.4ml/kg) given over 20 minutes
Inositol low volumeInositol lower volumeSingle dose of intravenous inositol 5%, 60 mg/kg (1.2ml/kg) given over 20 minutes
Placebo high volumePlacebo higher volumePlacebo (5% glucose) at a volume equal to 120 mg/kg (2.4 ml/kg) given via IV over 20 minutes
Placebo low volumePlacebo lower volumePlacebo (5% glucose) at a volume equal to 60 mg/kg (1.2 ml/kg) given via IV over 20 minutes.
Primary Outcome Measures
NameTimeMethod
Population pharmacokinetics0-100 hours following infusion
Secondary Outcome Measures
NameTimeMethod
Adverse events during and following infusion, using a neonatal toxicity classificationUntil discharge

Trial Locations

Locations (11)

Indiana University

🇺🇸

Indianapolis, Indiana, United States

University of Texas Southwestern Medical Center at Dallas

🇺🇸

Dallas, Texas, United States

Duke University

🇺🇸

Durham, North Carolina, United States

University of Utah

🇺🇸

Salt Lake City, Utah, United States

University of New Mexico

🇺🇸

Albuquerque, New Mexico, United States

Wayne State University

🇺🇸

Detroit, Michigan, United States

RTI International

🇺🇸

Durham, North Carolina, United States

Case Western Reserve University, Rainbow Babies and Children's Hospital

🇺🇸

Cleveland, Ohio, United States

Brown University, Women & Infants Hospital of Rhode Island

🇺🇸

Providence, Rhode Island, United States

University of Rochester

🇺🇸

Rochester, New York, United States

Yale University

🇺🇸

New Haven, Connecticut, United States

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