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Thrombosomes® in Bleeding Thrombocytopenic Patients Study

Phase 2
Terminated
Conditions
Hematologic Malignancy
Thrombocytopenia
Bone Marrow Aplasia
Myelodysplastic Syndromes
Myeloproliferative Disorders
Platelet Refractoriness
Interventions
Biological: Thrombosomes
Biological: Liquid Stored Platelets (LSP)
Registration Number
NCT04631211
Lead Sponsor
Cellphire Therapeutics, Inc.
Brief Summary

This prospective, multicenter, randomized, open-label, Phase 2, parallel, dose ranging, multidose trial will enroll patients into 3 Thrombosomes dose groups and 1 control liquid stored platelets (LSP) group in order to evaluate, in a dose-escalation manner, the safety, and impact on bleeding, and the preliminary effect on coagulation measures of increasing doses of allogeneic Thrombosomes in comparison to standard of care, LSP.

Detailed Description

Not available

Recruitment & Eligibility

Status
TERMINATED
Sex
All
Target Recruitment
21
Inclusion Criteria
  1. Adults (≥18 years) with TCP as defined by BOTH (a) and (b):

    1. a platlet count of ≤ 70,000 platelets/μL blood

    2. ANY ONE OR MORE of (1-3):

      1. confirmed diagnosis of hematologic malignancy, myeloproliferative disorder, myelodysplastic syndrome, or aplasia
      2. undergoing chemotherapy, immunotherapy, radiation therapy or hematopoietic stem cell transplantation
      3. refractory to platelet transfusion defined as two 1-hour CCI of <5,000 on consecutive transfusions of LSP or as defined by local site policy (Sacher, 2003)
  2. WHO Bleeding Score of 2 or 3

  3. Able to provide informed consent directly or through legally authorized representative, and comply with treatment and monitoring

  4. Negative pregnancy test for women of childbearing potential

Exclusion Criteria
  1. Any disorder or condition related to any venous thrombosis, embolism, or ischemia within the past 3 months
  2. Any disorder or condition related to arterial thrombosis including: ischemia, stroke, MI, or stent placement, within past 6 months
  3. Any valve replacement and/or repair of left atrial appendance occlusion device
  4. Sinusoidal obstruction syndrom (veno-occlusive disease) or cytopkine release syndrome associated with CAR-T cell therapy
  5. Refusal to accept blood products
  6. Liver enzyme blood levels greater than 3× the upper limit of normal (ULN)
  7. Blood creatinine level greater than 3× ULN
  8. Received platelet inhibitor drugs, cyclooxygenase-2 (COX-2) inhibitors, or nonsteroidal anti-inflammatory drugs within 5 days prior to infusion
  9. Currently (at the time of randomization) receiving anticoagulant therapy or antiplatelet therapy. Low dose prophylaxis for line clots is not excluded.
  10. Receipt of any pro-coagulant agents (e.g., DDAVP, recombinant Factor VIIa or prothrombin complex concentrates (PCC)) other than Tranexamic Acid (TXA) or Epsilon Aminocaproic Acid (EACA, Amicar), within 48 hours of first infusion, or with known hypercoagulable state
  11. WHO Bleeding Score of 2 solely due to lumbar puncture, retinal bleeding or GI bleeding or WHO Bleeding Score of 3 solely due to lumbar puncture
  12. Receiving L-asparaginase as part of a current cycle of treatment
  13. Known inherited or acquired bleeding disorder including, but not limited to: acquired storage pool deficiency or paraproteinemia with platelet inhibition
  14. Known inherited or acquired prothrombotic disorders, including antiphospholipid syndrome (Those with lupus anticoagulant or positive antiphospholipid serology without thrombosis are NOT excluded.)
  15. Anuria
  16. On dialysis
  17. Receipt of an investigational drug within 1 month before first infusion, other than for treatment of their underlying disease
  18. Females pregnant or nursing or unwilling to use contraception during and for 30 days after taking the study product (females). Evidence of effective birth control may be used, at the discretion of the physician
  19. Acute or chronic medical disorder that, in the opinion of the Investigator, would impair the ability of the patient to receive or respond to study treatment
  20. Prior participation in this study with successful infusion of the investigational or control product
  21. Currently enrolled in other trials not related to their primary disease process or involving platelet transfusions, platelet growth factors, or other pro-coagulant agents

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Thrombosomes Low DoseThrombosomes-
Thrombosomes Medium DoseThrombosomes-
Thrombosomes High DoseThrombosomes-
Liquid Stored Platelets (Control)Liquid Stored Platelets (LSP)-
Primary Outcome Measures
NameTimeMethod
Primary Efficacy EndpointEvaluated at 24 hours post initial infusion

Cessation or decrease in bleeding at primary bleeding site, based upon the most severe bleeding location at Day 1 baseline taken with in 12 hours prior to infusion, as evidenced by ordinal change in WHO (World Health Organization) Bleeding Score evaluated at 24 hours post initial infusion.

Secondary Outcome Measures
NameTimeMethod
Secondary Efficacy Endpoint assessed by Number of days alive and without WHO (World Health Organization) Grade 2a or greater bleeding7 days after first Thrombosomes or LSP infusion

Number of days alive and without WHO (World Health Organization) Grade 2 or greater bleeding through initial 7 days after first Thrombosomes or LSP Infusion

Secondary Efficacy Endpoint assessed for Number, timing, type and reason for administration of all blood products7 days after first Thrombosomes or LSP infusion

Number, timing, type and reason for administration of all blood products including platelets and Thrombosomes during the initial 7 days after first Thrombosomes or LSP infusion

Secondary Efficacy Endpoint assessed by 30 day mortality30 days post first infusion (+/- 2 days)

30-day mortality post first infusion of Thrombosomes or post first infusion of LSP as control

Secondary Efficacy Endpoint assessed by cessation or decrease in bleeding, as evidenced by ordinal change in WHO (World Health Organization) Bleeding Score24, 48, 72 hours, Day 4, Day 5, Day 6 and Day 7 post first infusion

Cessation or decrease in each additional bleeding site (other than primary bleeding site), as evidenced by ordinal change in WHO (World Health Organization) Bleeding Score at 24, 48, 72 hours, Day 4, Day 5, Day 6 and Day 7 after first infusion of Thrombosomes or LSP infusion as control.

Secondary Efficacy Endpoint assessed by measures of coagulationFrom baseline through last study visit (up to 30 days (+/- 2 days))

Measures of coagulation including: prothrombin time (PT); international normalized ratio (INR); fibrinogen; D-dimer; activated partial thromboplastin time (aPTT); and thromboelastography (TEG) or rotational thromboelastometry (ROTEM) per schedule of assessments

Secondary Efficacy Endpoint assessed by changes in markers of endothelial cell injury/repairFrom baseline through last study visit (up to 30 days (+/- 2 days))

Changes in markers of endothelial cell injury/repair from preinfusion baseline through 72 hours after first infusion, including: Syndecan-1, hyaluronan, thrombomodulin, vascular endothelial growth factor (VEGF), interleukin 6, sVE cadherin per schedule of assessments.

Secondary Efficacy Endpoint assessed by platelet count24, 48, 72 hours and Day 7 post first infusion

Platelet count measured at 24, 48, 72 hours and Day 7 of first infusion of Thrombosomes or LSP. Also evaluate at Day 4-6 if patient is hospitalized at that time.

Secondary Efficacy Endpoint assessed by measures of hematologyFrom baseline through last study visit (up to 30 days (+/- 2 days))

Measures of hematology including: Prothrombin Fragment 1+2; thrombin generation assay (TGA); Thrombopoietin; activated Protein C, tissue plasminogen activator (TPA), and plasminogen activator inhibitor (PAI) per schedule of assessments

Trial Locations

Locations (6)

City of Hope

🇺🇸

Duarte, California, United States

Helse Bergen Haukeland University Hospital

🇳🇴

Bergen, Norway

Medstar Georgetown

🇺🇸

Washington, District of Columbia, United States

Vanderbilt University Medical Center

🇺🇸

Nashville, Tennessee, United States

Rambam Medical Center

🇮🇱

Haifa, Israel

MD Anderson Cancer Center

🇺🇸

Houston, Texas, United States

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