Methylene Blue Intra-vital Nerve Staining
- Conditions
- Neurologic Findings
- Interventions
- Diagnostic Test: Methylene blue intradermal injection
- Registration Number
- NCT05547685
- Lead Sponsor
- National Taiwan University Hospital
- Brief Summary
In previous studies, we know methylene blue (MB) stains nerves, and oxidized MB has near-infrared (NIR) fluorescence for fluorescent observation. In this trial we using MB on human skin and combining MB and third-harmonic generation imaging (THG) to label cutaneous nerve fibers.
- Detailed Description
Intravital images were obtained before injection and then taken at 0.5, 1, 1.5, 2, 2.5, and 3 h after intradermal 0.03% (0.3 mg/mL, 1500μM) MB (10mg/mL "Astar") injection using a 0.5-mL 29-gauge insulin syringe needle (Becton, Dickinson and Company, USA). The participant lay down and the skin was imaged 10 cm above the ankle in the lower leg and above the wrist in the middle forearm; three sites in each limb were randomly selected for each time point. SHG or THG captured MB signals with two-photon excitation microscope at the Molecular Biomedical Imaging Research Center of National Taiwan University.
Recruitment & Eligibility
- Status
- UNKNOWN
- Sex
- All
- Target Recruitment
- 5
- Healthy Volunteers
- used serotonin agonist medication
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- SINGLE_GROUP
- Arm && Interventions
Group Intervention Description Methylene blue intradermal injection Methylene blue intradermal injection Methylene blue intradermal injection
- Primary Outcome Measures
Name Time Method intravital staining 12 months injected 10 μL of 0.03% methylene blue intradermally then calculate nerve fiber image
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
National Taiwan University Hosipital
🇨🇳Taipei City, Taiwan