Effects of Protein Supplementation on Skeletal Muscle Regeneration and Healing Process Following Exercise-induced Aseptic Injury
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 14
- 试验地点
- 2
- 主要终点
- Changes in volume and morphological complexity of immune cells
研究概览
简要总结
In this study the investigators utilized protein supplementation over an 8-day period following eccentric exercise-induced muscle damage in order to test the initial hypotheses : i) protein supplementation after exercise-induced muscle injury affects exercise-induced aseptic inflammation and muscle performance.
详细描述
The objective was to examine weather protein supplementation is able to affect the inflammatory response as well as recovery of muscle performance following an intense eccentric exercise protocol. In a double-blind, counterbalanced design, 14 men received either Placebo (PLA) or milk protein isolate (PRO) for 8 consecutive days following a single bout of exercise (300 eccentric contractions at 30 deg/sec). In both conditions, performance was assessed at baseline, immediately post-exercise, 2h post-exercise and daily for 8 consecutive days. Blood samples were collected at baseline, 2h post-exercise and daily for the remaining 8 days. Muscle biopsies from vastus lateralis were collected at baseline as well as at day 2 and day 8 of the post-exercise period.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Double (Participant, Investigator)
入排标准
- 年龄范围
- 18 Years 至 30 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •a) recreationally trained as indicated by the maximal oxygen consumption levels (VO2max > 45 ml/kg/min), b) engaged in systematic exercise at least three times per week for > 12 months, c) non-smokers, d) abstained from any vigorous physical activity during the study, e) abstained from consumption of caffeine, alcohol, performance-enhancing or antioxidant supplements, and medications during the study.
排除标准
- •a) a recent febrile illness, b) history of muscle lesion, c) lower limb trauma
结局指标
主要结局
Changes in volume and morphological complexity of immune cells
时间窗: 1h before exercise, 2h post-exercise, daily for 8 days post-exercise
Change in proteasome activities in muscle
时间窗: 1h before exercise, 2 days post-exercise, 8 days post-exercise
Measurement of LLVY, LSTR and LLE
Change in creatine kinase activity in plasma
时间窗: 1h before exercise, 2h post-exercise, daily for 8 days post-exercise
Change in neutrophil count in blood
时间窗: 1h before exercise, 2h post-exercise, daily for 8 days post-exercise
Change in cytokine concentration in plasma
时间窗: 1h before exercise, 2h post-exercise, daily for 8 days post-exercise
Measurement of IL-1β, IL-4, IL-6, IL-8, IL-10, TNF-α
Change in total antioxidant capacity in serum
时间窗: 1h before exercise, 2h post-exercise, daily for 8 days post-exercise
Total antioxidant capacity in serum
Change in testosterone concentration in plasma
时间窗: 1h before exercise, 2h post-exercise, daily for 8 days post-exercise
Change in protein expression level of proteasome subunits
时间窗: 1h before exercise, 2 days post-exercise, 8 days post-exercise
Measurement of B1i, B2i, B5i, B5, B1, B2 and α7
Change in protein carbonyls in serum
时间窗: 1h before exercise, 2h post-exercise, daily for 8 days post-exercise
Concentration of protein carbonyls
Change in thiobarbituric acid and reactive substances in serum
时间窗: 1h before exercise, 2h post-exercise, daily for 8 days post-exercise
Thiobarbituric acid reactive substances concentration in serum
Change in white blood cell count in blood
时间窗: 1h before exercise, 2h post-exercise, daily for 8 days post-exercise
Change in glucose concentration in blood
时间窗: 1h before exercise, 2h post-exercise, daily for 8 days post-exercise
Change in insulin concentration in blood
时间窗: 1h before exercise, 2h post-exercise, daily for 8 days post-exercise
Change in adhesion molecule concentration in blood
时间窗: 1h before exercise, 2h post-exercise, daily for 8 days post-exercise
Change in reduced glutathione in blood
时间窗: 1h before exercise, 2h post-exercise, daily for 8 days post-exercise
Concentration of reduced glutathione in red blood cells
Change in protein carbonyls in muscle
时间窗: 1h before exercise, 2 days post-exercise, 8 days post-exercise
Protein carbonyl concentration in quadriceps skeletal muscle group
Change in oxidized glutathione in blood
时间窗: 1h before exercise, 2h post-exercise, daily for 8 days post-exercise
Concentration of oxidized glutathione in red blood cells
Change in catalase activity in serum
时间窗: 1h before exercise, 2h post-exercise, daily for 8 days post-exercise
Catalase activity in serum
Change in C-reactive protein in plasma
时间窗: 1h before exercise, 2h post-exercise, daily for 8 days post-exercise
Change in intracellular signalling proteins in muscle
时间窗: 1h before exercise, 2 days post-exercise, 8 days post-exercise
Measurement of phosphorylation levels of mammalian target of rapamycin (mTOR), ribosomal protein S6 (rpS6) and nuclear factor kB (NFkB), and protein expression levels of forkhead box protein O1 (FOXO1), HSP70, and parkin.
次要结局
- Maximal aerobic capacity(One day before exercise)
- Body composition(One day before exercise)
- Change in muscle function of knee extensor and flexor muscle(1h before exercise, 5 min post-exercise, 2h post-exercise, daily for 8 days post-exercise)
- Change in dietary intake profile(1h before exercise, daily for 8 days post-exercise)
研究者
Ioannis G. Fatouros
Associate Professor
University of Thessaly