Influence of preservation environments on salivary microbiota profiles in 16S rRNA sequencing
Not Applicable
- Conditions
- Healthy subjects
- Registration Number
- JPRN-UMIN000043823
- Lead Sponsor
- The Jikei University School of Medicine
- Brief Summary
An almost perfect correlation was shown between the immediate extraction and the -15 degree storage group for relative abundance at the genus and operational taxonomic unit levels. The intraindividual UniFrac distances among storage methods were significantly shorter than those of interindividual distances. None of the amount of extracted DNA, the alfa-diversity indices, or the relative abundance at the phylum/genus/operational taxonomic unit level differed among storage methods.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Complete: follow-up complete
- Sex
- All
- Target Recruitment
- 5
Inclusion Criteria
Not provided
Exclusion Criteria
1. those whose salivary samples were not or could not be stored in the respective storage environments 2. those who refused to participate by opting out
Study & Design
- Study Type
- Observational
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Microbial profiles based on 16S rRNA amplicon sequencing
- Secondary Outcome Measures
Name Time Method