Investigation of Stem Cells in Human Lipoaspirate

• Conditions: Breast Cancer

• Registration Number: NCT01677520
• Lead Sponsor: University of Nevada, Las Vegas

Brief Summary

The overall purpose of this study is to enhance identification and isolation of ADSCs and adipocytes, to find the optimum culturing conditions to increase growth and volume of grafts, and to study the effects of cryopreservation on ADSCs and adipocytes. Fat injections are used in aesthetic surgery but tend to disassemble over time. If the investigators can enhance growth and volume of fat in culture, the investigators could use fat grafts in reconstruction of cancer and traumatic injury defects with the patients own cultured fat cells and grafts.

Detailed Description

Not available

Study Timeline

• Study Start: 2011-03
• Study First Submitted: 2012-08-28
• Study First Submitted QC: 2012-08-31
• Study First Posted: 2012-09-03
• Status Verified: 2015-01
• Last Update Submitted: 2015-01-08
• Last Update Posted: 2015-01-09
• Primary Completion: 2018-08
• Study Completion: 2018-08

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 240

Inclusion Criteria

• All adults (18-70 yrs) who have scheduled surgical lipectomy of the hips, abdomen, or thighs are prospective subjects for this study.

Exclusion Criteria

• Pregnant women will be excluded to protect the unborn child.

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Percent of Viable ADSCs	Day of Surgery	Percent of viable ADSCs will be measured on fresh samples and samples refrigerated for different times.

Secondary Outcome Measures

Name	Time	Method
Percent of Apoptotic ADSCs	Day of Surgery	Percent of apoptotic ADSCs will be measured on fresh samples and on refrigerated samples at 1, 2, 3, and 4 days refrigerated storage.

Trial Locations

Locations (1)

University of Nevada School of Medicine Patient Care Center; 🇺🇸 Las Vegas, Nevada, United States