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Pomegranate Extract Supplementation in Colorectal Cancer Patients

Phase 1
Completed
Conditions
Colorectal Cancer
Interventions
Dietary Supplement: Standard pomegranate extract formulation
Dietary Supplement: Pomegranate extract formulation-1
Dietary Supplement: Pomegranate extract formulation-2
Registration Number
NCT01916239
Lead Sponsor
National Research Council, Spain
Brief Summary

The most relevant pomegranate phenolics (ellagitannins and ellagic acid) are extensively metabolized by the human gut microbiota to yield a number of metabolites called urolithins (mainly Uro-A). Urolithins have been reported to regulate in vivo the expression of genes involved in inflammation and cancer. Our hypothesis is that urolithins can be detected in the human colon mucosa where these metabolites can exert anti-inflammatory and anti-cancer activities. After colonoscopy and diagnosis, colorectal cancer patients will consume capsules containing three different pomegranate extract formulations until surgery. The aims of this trial are:

* To evaluate the disposition of pomegranate phenolics and urolithins in tumoral and normal colon tissues.

* To evaluate gene expression profiling and protein markers in tumoral and normal colon tissues from these patients.

* To compare different pomegranate extract formulations on the above.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
60
Inclusion Criteria
  • Colorectal cancer diagnosis.
  • Surgery required.
  • WHO status: between 0 and 2.
  • Hemoglobin >10 g/dL
  • ALT >2.5-fold above the normal value.
  • Serum Bilirubin >1.5-fold above the normal value.
  • Creatinine <140 micromol/L
Exclusion Criteria
  • Patients who do not satisfy inclusion criteria and,
  • Active pectic ulcer.
  • Pregnancy or breastfeeding.
  • Alcoholism.
  • Chemotherapy or radiotherapy a month prior to recruitment.
  • Treatment with steroids or other anti-inflammatory drugs a week prior to recruitment.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Standard pomegranate extract formulationStandard pomegranate extract formulationStandard pomegranate extract formulation containing 20% punicalagin
Pomegranate extract formulation-1Pomegranate extract formulation-1New pomegranate extract formulation-1
Pomegranate extract formulation-2Pomegranate extract formulation-2New pomegranate extract formulation-2
Primary Outcome Measures
NameTimeMethod
Phenolics and derived metabolites in colon tissues, plasma and urine.Change from baseline at 15 days

Occurrence of phenolics and gut-microbiota derived metabolites in tumoral and colon tissues, urine and plasma.

Gene expression profiling in colon tissuesChange from baseline at 15 days

Gene expression profile changes in tumoral and normal colon tissues

Secondary Outcome Measures
NameTimeMethod
IGF-1 (insulin-like growth factor-1)Change from baseline at 15 days

Change in circulating IGF-1 levels

CEA (carcnoembryonic antigen)Change from baseline at 15 days

Change in circulating CEA levels

Number of patients with adverse events as a measure of safety and tolerabilityChange from baseline at 15 days

* Change in markers involved in hepatic and renal functions: GGT, AST, ALP, ALT, CPK, urate, creatinin, albumin, bilirubin, LDH.

* Change in hematological variables: leucocytes, neutrophils, lymphocytes, monocytes, eosinophils, basophils, hemoglobin, hematocrit, mean corpuscular volume, mean platelet volume, platelets, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration.

* Intolerance, dyspepsia, allergic reactions, constipation, diarrhea, abdominal pain, nausea.

microRNA expression profiling in colon tissuesChange from baseline at 15 days

microRNA (miR) expression profile change in tumoral and normal colon tissues

Trial Locations

Locations (1)

Hospital General Universitario Reina Sofía

🇪🇸

Murcia, Spain

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