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Microbiome and Host Susceptibility in Severe Pneumonia, a Prospective, Multicenter, Cohort Study

Recruiting
Conditions
Severe Pneumonia
Interventions
Diagnostic Test: metagenomics
Registration Number
NCT06114784
Lead Sponsor
First Affiliated Hospital of Zhejiang University
Brief Summary

This study was a prospective multicenter cohort of severe pneumonia. And by collecting clinical samples to clarify the correlation between lung microbiome, intestinal microbiome, host susceptibility, and prognosis of severe pneumonia patients.

Detailed Description

The human lung and intestinal microbiota play an important role in human health. At present, the correlation between lung and intestinal microbiota in severe pneumonia patients and host susceptibility is limited to small sample, single center studies. Due to sample size limitations, the pathogenesis of severe pneumonia caused by many pathogens remains unclear. This study plans to construct sputum, alveolar lavage fluid, feces, and whole blood samples of severe pneumonia patients admitted to the ICU from 2023 to 2025, and collect clinical data from patients to identify changes in lung and intestinal microbiome, host susceptibility, and disease progression risk in different groups of patients.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
2000
Inclusion Criteria
  1. New lung infection that meets one of the following definitions: the patient receives mechanical ventilation (invasive or non-invasive) due to acute respiratory failure, with a PEEP level of 5cm or above; Patients receiving high flow oxygen therapy with a FiO2 of 50% or more and a PaO2: FiO2 ratio below 300
Exclusion Criteria
  1. Expected length of stay in ICU is less than 1 day
  2. Pregnant

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
Severe pneumoniametagenomicsDefinition of severe pneumonia: New infection that meets one of the following definitions: the patient receives mechanical ventilation (invasive or non-invasive) due to acute respiratory failure, with a PEEP level of 5cm or above; The patient receives high flow oxygen therapy with a FiO2 of 50% or more and a PaO2: FiO2 ratio of less than 300; The patient receives oxygen treatment with a partial respiration mask with an air storage bag, provided that PaO2 is lower than the pre-specified indicator.
Primary Outcome Measures
NameTimeMethod
The correlation between lung microbiome and disease prognosis60 days

1. Identification of microbial gene profiles; 2. Analysis of host health dynamics and trends; 3. Identification of host gene mutations; 4. Preliminary model construction; 5. Model optimization and evaluation.

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (15)

The Second Affiliated Hospital of Zhejiang University School of Medicine

🇨🇳

Hangzhou, Zhejiang, China

First Affiliated Hospital of Zhejiang University School of Medicine

🇨🇳

Hangzhou, Zhejiang, China

Shulan (Hangzhou) hospital

🇨🇳

Hangzhou, China

Tongde Hospital of Zhejiang Province

🇨🇳

Hangzhou, China

the First People's Hospital of Huzhou

🇨🇳

Huzhou, China

The First Hospital of Jiaxing

🇨🇳

Jiaxing, China

Lanxi Hospital of Traditional Chinese Medicine

🇨🇳

Lanxi, China

Lishui People's Hospital

🇨🇳

Lishui, China

The First People's Hospital of Pinghu

🇨🇳

Pinghu, China

Taizhou Hospital of Zhejiang Province affiliated to Wenzhou Medical University

🇨🇳

Taizhou, China

Wenzhou Central Hospital

🇨🇳

Wenzhou, China

Xi'an People's Hospital (Xi'an No.4 Hospital)

🇨🇳

Xi'an, China

Henan Provincial People's Hospital

🇨🇳

Zhengzhou, China

The Fifth Clinical Medical College of Henan University of Chinese Medicine

🇨🇳

Zhengzhou, China

The Second Affiliated Hospital of Zhengzhou University

🇨🇳

Zhengzhou, China

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