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The Effect and Safety Profile of Thymoglobulin® in Primary Cardiac Transplant Recipients

Phase 2
Conditions
Heart Transplantation
Interventions
Registration Number
NCT03292861
Lead Sponsor
Cedars-Sinai Medical Center
Brief Summary

This is a randomized, controlled, single center study to evaluate the efficacy of Thymoglobulin induction therapy in combination with Mycophenolate Mofetil, tacrolimus, and steroids in the prevention of CAV. Approximately half of the patients will be randomized to receive a total of 5 doses of Thymoglobulin during the study. The first dose of Thymoglobulin will be administered at 1.5 mg/kg via intravenous infusion over 6 hours immediately upon arrival to the ICU post-operation (day 1). Subsequent doses of 1.5 mg/kg will be administered on days 2, 3, 4, and 5 via IV infusion over 4 hours.

Mechanistic assays (T-reg cells, Lym subsets, B cell subsets, IL-1b, cytokines, TGFb, IL-21 to be drawn at Pre-transplant, 3, 6, 12 months post-transplant) will also be performed.

All patients will be followed and monitored according to standard of care protocols for heart transplant recipients at our center.

Detailed Description

Not available

Recruitment & Eligibility

Status
ENROLLING_BY_INVITATION
Sex
All
Target Recruitment
60
Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Thymoglobulin®CorticosteroidsThymoglobulin® (Genzyme) \[rabbit anti-thymocyte globulin (ATG)\] is a purified pasteurized, gamma immune globulin, obtained by immunization of rabbits with human thymocytes. This immunosuppressive product contains polyclonal cytotoxic antibodies directed against antigens expressed on human T-lymphocytes. Approximately half of the patients will be randomized to receive a total of 5 doses of Thymoglobulin during the study. The first dose of Thymoglobulin will be administered at 1.5 mg/kg via intravenous infusion over 6 hours immediately upon arrival to the ICU post-operation (day 1). Subsequent doses of 1.5 mg/kg will be administered on days 2, 3, 4, and 5 via IV infusion over 4 hours. In addition, there will be maintenance doses of mycophenolate mofetil, tacrolimus, sirolimus, and cumulative dose of corticosteroids at 12 months post-transplantation
Thymoglobulin®ThymoglobulinThymoglobulin® (Genzyme) \[rabbit anti-thymocyte globulin (ATG)\] is a purified pasteurized, gamma immune globulin, obtained by immunization of rabbits with human thymocytes. This immunosuppressive product contains polyclonal cytotoxic antibodies directed against antigens expressed on human T-lymphocytes. Approximately half of the patients will be randomized to receive a total of 5 doses of Thymoglobulin during the study. The first dose of Thymoglobulin will be administered at 1.5 mg/kg via intravenous infusion over 6 hours immediately upon arrival to the ICU post-operation (day 1). Subsequent doses of 1.5 mg/kg will be administered on days 2, 3, 4, and 5 via IV infusion over 4 hours. In addition, there will be maintenance doses of mycophenolate mofetil, tacrolimus, sirolimus, and cumulative dose of corticosteroids at 12 months post-transplantation
Thymoglobulin®Mycophenolate MofetilThymoglobulin® (Genzyme) \[rabbit anti-thymocyte globulin (ATG)\] is a purified pasteurized, gamma immune globulin, obtained by immunization of rabbits with human thymocytes. This immunosuppressive product contains polyclonal cytotoxic antibodies directed against antigens expressed on human T-lymphocytes. Approximately half of the patients will be randomized to receive a total of 5 doses of Thymoglobulin during the study. The first dose of Thymoglobulin will be administered at 1.5 mg/kg via intravenous infusion over 6 hours immediately upon arrival to the ICU post-operation (day 1). Subsequent doses of 1.5 mg/kg will be administered on days 2, 3, 4, and 5 via IV infusion over 4 hours. In addition, there will be maintenance doses of mycophenolate mofetil, tacrolimus, sirolimus, and cumulative dose of corticosteroids at 12 months post-transplantation
Thymoglobulin®TacrolimusThymoglobulin® (Genzyme) \[rabbit anti-thymocyte globulin (ATG)\] is a purified pasteurized, gamma immune globulin, obtained by immunization of rabbits with human thymocytes. This immunosuppressive product contains polyclonal cytotoxic antibodies directed against antigens expressed on human T-lymphocytes. Approximately half of the patients will be randomized to receive a total of 5 doses of Thymoglobulin during the study. The first dose of Thymoglobulin will be administered at 1.5 mg/kg via intravenous infusion over 6 hours immediately upon arrival to the ICU post-operation (day 1). Subsequent doses of 1.5 mg/kg will be administered on days 2, 3, 4, and 5 via IV infusion over 4 hours. In addition, there will be maintenance doses of mycophenolate mofetil, tacrolimus, sirolimus, and cumulative dose of corticosteroids at 12 months post-transplantation
Thymoglobulin®SirolimusThymoglobulin® (Genzyme) \[rabbit anti-thymocyte globulin (ATG)\] is a purified pasteurized, gamma immune globulin, obtained by immunization of rabbits with human thymocytes. This immunosuppressive product contains polyclonal cytotoxic antibodies directed against antigens expressed on human T-lymphocytes. Approximately half of the patients will be randomized to receive a total of 5 doses of Thymoglobulin during the study. The first dose of Thymoglobulin will be administered at 1.5 mg/kg via intravenous infusion over 6 hours immediately upon arrival to the ICU post-operation (day 1). Subsequent doses of 1.5 mg/kg will be administered on days 2, 3, 4, and 5 via IV infusion over 4 hours. In addition, there will be maintenance doses of mycophenolate mofetil, tacrolimus, sirolimus, and cumulative dose of corticosteroids at 12 months post-transplantation
Primary Outcome Measures
NameTimeMethod
Percentage of Participants With Composite Efficacy Failure at 12 Months12 Months

Composite efficacy failure is defined as development of de novo donor specific antibodies (measured by standardized alloantibody testing by Luminex and control sera, characterizing kinetics, specificities and relative binding strength) and ischemia on endomyocardial biopsy (characterized on biopsy by myocyte necrosis and/or regions of myocyte dropout) at 12 months post-transplant.

Secondary Outcome Measures
NameTimeMethod
Changes in biomarkers12 months

Correlations between significant changes in biomarkers and their relation to any significant differences in clinical outcomes

Number of patients who experience rejection12 months

Number of patients who experience acute cellular, antibody-mediated, hemodynamic compromise, and any-treated rejection within first 12 months after transplantation/

First rejection by ISHLT biopsy grading scale12 months

First acute cellular, antibody-mediated, hemodynamic compromise, and any-treated rejection by the ISHLT biopsy grading scale in the first 12 months post-transplantation

Time to first rejection12 months

Time to first acute cellular, antibody-mediated, hemodynamic compromise, and any-treated rejection within the first 12 months

Incidence of primary graft dysfunction (PGD)first 24 hours post-transplant

The incidence of Primary Graft Dysfunction(PGD) in the first 24 hours post-transplant

Patient and graft survival12 months

Patient and graft survival at 12 months post-transplantation

Types of patients with fatal infectious complications12 months

The types of patients with fatal infectious complications (especially CMV infection) within the first 12 months post-transplantation

Number of patients with fatal infectious complications12 months

The number of patients with fatal infectious complications (especially CMV infection) within the first 12 months post-transplantation

Types of patients with non-fatal infectious complications12 months

Types of patients with non-fatal infectious complications (especially CMV infection) within the first 12 months post-transplantation

Number of patients with non-fatal infectious complications12 months

Number of patients with non-fatal infectious complications (especially CMV infection) within the first 12 months post-transplantation

Freedom from development of circulating antibodies12 months

Freedom from the development of circulating antibodies within the first 12 months post transplantation, where circulating antibodies include donor specific antibodies (DSA), non-specific antibodies, and non-human leukocyte antigen antibodies

Change in coronary maximal intimal thickness12 months

Change in coronary maximal intimal thickness at matched sites by intravascular ultrasound at 12 months

Changes in immune cell profiles12 months

Correlations between significant changes in immune cell profiles and biomarkers, and their relation to any significant differences in clinical outcomes

Number of episodes per patient12 months

Number of acute cellular, antibody-mediated, hemodynamic compromise and any-treated rejection episodes per patient within the first 12 months post-transplantation

Change in coronary intimal area12 months

Change in coronary intimal area at matched sites by intravascular ultrasound at 12 months

Change in coronary intimal volume12 months

Change in coronary intimal volume at matched sites by intravascular ultrasound at 12 months

Change in coronary vessel area12 months

Change in coronary vessel area at matched sites by intravascular ultrasound at 12 months

Change in coronary intimal index12 months

Change in coronary intimal index at matched sites by intravascular ultrasound at 12 months

Change in coronary percent atheroma volume12 months

Change in coronary percent atheroma volume at matched sites by intravascular ultrasound at 12 months

Maintenance doses of mycophenolate mofetil, tacrolimus, sirolimus, and dose of corticosteroids12 months

Maintenance doses of mycophenolate mofetil, tacrolimus, sirolimus, and cumulative dose of corticosteroids at 12 months post-transplantation

Number of hospital days per patient3 months, 6 months, 12 months

Number of hospital days per patient, both during the transplant period and during the post-transplant period at 3 months, 6 months, and 1 year

Number of patients requiring hospitalization3 months, 6 months, 12 months

Number of patients requiring hospitalization by 3 months, by 6 months, or by 1 year post-transplantation

Death/Re-transplant12 months

To describe between treatment groups the incidence of the composite primary endpoint of death/re-transplant at 12 months post-transplantation

Hemodynamic compromise rejection12 months

To describe between treatment groups the incidence of the composite primary endpoint of hemodynamic compromise rejection at 12 months post-transplantation. This is an ejection fraction of ≤ 30% or a 0.20 absolute decrease from baseline, and the need for inotropic agents OR a fractional shortening ≤ 20% or a 25% decrease from baseline, and the need for inotropic agents PLUS need for inotropic agents due to a Cardiac Index (CI) \< 2.0 L/min/m2 or a 25% decrease from baseline

Graft dysfunction12 months

To describe between treatment groups the incidence of the composite primary endpoint of graft dysfunction (ejection fraction less than or equal to 40% by echocardiography)at 12 months post-transplantation

Cellular rejection12 months

To describe between treatment groups the incidence of the composite primary endpoint of biopsy proven cellular rejection ≥2R at 12 months post-transplantation

Antibody mediated rejected12 months

To describe between treatment groups the incidence of the composite primary endpoint of biopsy proven antibody mediated rejection ≥AMR1 at 12 months post-transplantation

Cardiac Allograft Vasculopathy (CAV)12 months

To describe between treatment groups the incidence of cardiac allograft vasculopathy (CAV) (defined as a change ≥0.5mm in maximal intimal thickness (MIT) of the coronary arteries by intravascular ultrasound at 12 months as compared to baseline)

Any treated rejection12 months

To describe between treatment groups any treated rejection at 12 months

Trial Locations

Locations (2)

Cedars-Sinai Medical Center

🇺🇸

Los Angeles, California, United States

Kaiser Permanente Los Angeles Medical Center

🇺🇸

Los Angeles, California, United States

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