Stem Cell Isolation From Femoral Heads Post Total Hip Arthroplasty

Not Applicable

Not yet recruiting

• Conditions: Obesity and Type 2 Diabetes

• Registration Number: NCT06775600
• Lead Sponsor: David Hess

Brief Summary

Femoral heads will be collected at University Hospital (London, ON, Canada) from individuals with type-2 diabetes, obesity, and no T2D/no obesity controls. The femoral head will be taken to the Robarts Research Institute (London, ON, Canada) for lineage-restricted progenitor cell expansion and analyses. There the femoral heads will be used to isolate three stem cell types - Multipotent Stromal Cells, Endothelial Colony Forming Cells, and Hematopoietic Progenitor Cells. The yield and cell surface markers expressed on these cells will be measured using flow cytometry. These cell types will then undergo further analyses of the secretome, tubule forming assays, vascular regenerative cell exhaustion assays, and will be transplanted into immune deficient mice with with femoral artery ligation. These experiments are to assess the differences in function and expression of these cell types between individuals with type-2 diabetes and/or obesity and/or healthy controls.

Detailed Description

Femoral Heads will be extracted by Dr. Lanting (Department of Surgery - Division of Orthopaedics) at University Hospital, they will then be transferred to Robarts Research Institute where they will undergo cell isolation and analyses. Multipotent Stromal Cells, Endothelial Colony Forming Cells, and Hematopoietic Progenitor Cells will be extracted from the bone marrow of the femoral heads. These cells will be used to characterize the difference in regenerative progenitor cell number and potential dysfunction in individuals with type 2 diabetes and/or obesity compared to controls. Analysis will include growth kinetics, flow cytometry, Bio Luminescent Imaging and Laser Doppler Perfusion Imaging using a FAL mouse model.

Study Timeline

• Status Verified: 2025-01
• Study First Submitted: 2025-01-09
• Study First Submitted QC: 2025-01-14
• Last Update Submitted: 2025-01-14
• Study First Posted: 2025-01-15
• Last Update Posted: 2025-01-15
• Study Start: 2025-02-01
• Primary Completion: 2025-05-01
• Study Completion: 2025-08-01

Recruitment & Eligibility

• Status: NOT_YET_RECRUITING
• Sex: All
• Target Recruitment: 30

Inclusion Criteria

• Must be 18+ years of age

Exclusion Criteria

• Diagnosis of bone cancers
• Diagnosis of Rheumatoid arthritis
• Diagnosis of hematopoietic conditions
• Diagnosis of avascular necrosis of the hip
• does not fall within BMI requirements for experimental groups
• Cannot read and/or speak in english

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Primary Outcome Measures

Name	Time	Method
Cell Yield	Within 24 hours of surgery	Quantification of how many cells are harvested from each femoral head prior to expansion in vitro
Cellular Growth Kinetics	Time of cell harvest to 8 weeks post harvest	Quantification of the Growth Kinetics of each cell type during in vitro cell culture post cell harvest.
Cellular Phenotype	Time of cell harvest to 8 weeks post harvest	Analysis via flow cytometry of the phenotype of each cell type and their respective surface markers that are involved in regenerative pathways.