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Resistance Training and Hydrolyzed Collagen Supplementation in Middle-aged Adults

Not Applicable
Completed
Conditions
Diet
Food
Nutrition
Interventions
Dietary Supplement: Resistance training with collagen supplementation
Registration Number
NCT06402890
Lead Sponsor
Liverpool John Moores University
Brief Summary

The aim of this clinical trial is to investigate the longitudinal effects of hydrolyzed collagen ingestion combined with resistance training on muscle-tendon unit structure and function in middle-aged males and females.

The main research questions this clinical trial aims to answer are:

1. Does resistance training with hydrolyzed collagen ingestion lead to greater changes in tendon properties than resistance training alone in middle-aged health men and women?

2. Does resistance training with hydrolyzed collagen ingestion lead to greater changes in muscle size than resistance training alone?

3. Does resistance training with hydrolyzed collagen lead to greater increases in strength and power compared to resistance training alone in middle-aged, healthy men and women?

Participants will be randomly assigned to collagen or placebo groups. Participants will perform 24 sessions of high intensity resistance training across 12-weeks. Alongside each training session, participants will consume a beverage containing hydrolyzed collagen or maltodextrin, with both beverages containing vitamin C.

Researchers will compare the collagen and placebo groups to see if there would be beneficial effects on changes in muscle and tendon that are greater than resistance training alone. To achieve this, an dynamometry will be used to assess lower limb strength and ultrasound will be used to measure the morphological, mechanical, and material properties of the patellar tendon, as well the size and architecture of the vastus lateralis muscle.

Detailed Description

Healthy, active, middle-aged men and women will ingest a beverage containing 30 g of hydrolyzed collagen with 50 mg of vitamin C or a calorie matched beverage (maltodextrin), also with 50 mg of vitamin C combined with high-volume, high-intensity resistance training for the lower body on 2 - 3 occasions per week for 8-12 weeks.

The aim of this study was to investigate the effect of combining hydrolyzed collagen with resistance training in middle-aged men and women.If supplementation with hydrolyzed collagen leads to a greater change in tendon size, stiffness, and Young's modulus than resistance training alone, this will allow us to recommend this type of intervention to young athletes seeking to improve tendon health and/or athletic performance. This is the first study to investigate the combination of hydrolyzed collage supplementation with resistance training in middle-aged men and women. If supplementation with hydrolyzed leads to greater improvements in tendon size, stiffness, strength and/or power, this will allow us to recommend this type of intervention to middle-aged athletes/trainees seeking to improve tendon health, reduce injury risk, or enhance athletic performance.

The experimental design of both arms will be the same, however Arm 1 will be health, middle-aged male participants and Arm 2 will be health, middle-aged female participants.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
42
Inclusion Criteria
  • Recreationally active or competing in Masters sport
  • No history of patellar tendon injuries in the past 6 months
  • No history of lower limb musculoskeletal injuries in the past 6 months
  • Non-smokers (including e-cigarettes)
  • Free from cardiovascular and metabolic diseases
Exclusion Criteria
  • Age < 35 years or > 59 years
  • Being vegan or vegetarian (due to the mammalian source of collagen)
  • Injury history incompatible with lower body resistance training
  • Consumption of nutritional supplementation that purportedly affects muscle-tendon adaptation or recovery (i.e. protein powder, vitamin C, collagen)
  • BMI over 30 kg/m2

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Resistance training with hydrolyzed collagen in health, active, middle-aged menResistance training with collagen supplementationMiddle-aged, trained males completed 12-weeks of a supervised, progressive resistance training programme while consuming 30 g of hydrolyzed collagen or a calorie matched placebo beverage.
Resistance training with hydrolyzed collagen in healthy, active, middle-aged womenResistance training with collagen supplementationMiddle-aged, trained females completed 8-weeks of a supervised, progressive resistance training programme while consuming 30 g of hydrolyzed collagen or a calorie matched placebo beverage
Primary Outcome Measures
NameTimeMethod
Knee extensor rate of torque development (in Newton metres per second)8-12 weeks' training and nutrition intervention

Changes in explosive knee extension strength were measured using isometric dynamometry (Biodex System 3, IPRS Mediquipe Limited, Lancashire, UK)

m. vastus lateralis thickness (in millimetres)8-12 weeks' training and nutrition intervention

Ultrasonography was used to determine changes in muscle thicknessprotocols

Barbell back squat 10-repetition maximum (in kilograms)8-12 weeks' training and nutrition intervention

Changes in dynamic lower body strength were measured using the maximum load during 10 repetitions of the barbell back squat exercise with standardised technique

m. vastus lateralis fascicle pennation angle (in degrees)8-12 weeks' training and nutrition intervention

Ultrasonography was used to determine the pennation angle of fascicles as they insert into the aponeurosis

Patellar tendon stiffness (in Newtons per millimetre)8-12 weeks' training and nutrition intervention

Synchronized ultrasonography, dynamometry, and surface electromyography were used to determine changes in patellar tendon stiffness

Knee extensor maximal isometric torque (in Newton metres)8-12 weeks' training and nutrition intervention

Changes in maximal knee extension strength were measured using isometric dynamometry (Biodex System 3, IPRS Mediquipe Limited, Lancashire, UK)

20-metre sprint time (in seconds)8-12 weeks' training and nutrition intervention

Double photocell timing gates (Witty System, Microgate SRL, Bolzano, Italy) were used to measure changes in time taken to cover 20-metres during an all out sprint on a standardised surface

Patellar tendon cross sectional area (in squared millimetres) at 3 regions along the tendon length8-12 weeks' training and nutrition intervention

Ultrasonography was used to determine changes in tendon cross sectional area at 25, 50, and 75 % of tendon length

Bilateral vertical countermovement jump height (in centimetres)8-12 weeks' training and nutrition intervention

Changes in jump height were estimated by use of the Optojump system (Microgate, Bolzano, Italy) and force plates (Force Decks Dual Force Plate System, VALD, Charlotte NC, USA).

Bilateral horizontal broad jump distance (in centimetres)8-12 weeks' training and nutrition intervention

Changes in maximal horizontal displacement were measured as the maximum distance covered during a broad jump on standardised surface without footwear

Lower limb maximal isometric force (in Newtons)8-12 weeks' training and nutrition intervention

Force plates (Force Decks Dual Force Plate System, VALD, Charlotte NC, USA) were used to measure changes in maximal multi-joint isometric strength during the mid-thigh pull exercise

Lower limb isometric rate of force development (in Newtons per second)8-12 weeks' training and nutrition intervention

Force plates (Force Decks Dual Force Plate System, VALD, Charlotte NC, USA) were used to measure changes in explosive multi-joint isometric strength during the mid-thigh pull exercise

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Dr Rob Erskine

🇬🇧

Liverpool, United Kingdom

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