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Effects of E-smoke on Levels of Endothelial Progenitor Cells and Microparticles in Healthy Volunteers

Not Applicable
Completed
Conditions
Healthy
Interventions
Device: Electronic cigarette
Registration Number
NCT02532894
Lead Sponsor
Karolinska Institutet
Brief Summary

We plan to investigate the acute effects of inhaling e-cigarette vapor on cell function measured by microvesicles and endothelial progenitor cells. Micro vesicles are released upon either activation or apoptosis from different cell types such as platelets, leucocytes and endothelial cell. Endothelial progenitor cells are a type of stem cells that circulate in the blood with the ability to differentiate to endothelial cells. Endothelial progenitor cells are inversely correlated to cardiovascular risk factors.

As a secondary endpoint we plan to investigate exhaled nitric oxide - a common inflammation marker used in asthmatic patients - after inhalation of electronic cigarette vapor.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
16
Inclusion Criteria
  • healthy subjects
  • sporadic cigarette smoking (<9 cigarettes per month)
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Exclusion Criteria
  • Any form of cardiovascular disease
  • Any form of pulmonary disease like asthma or COPD
  • Any form of systemic or chronic disorder like rheumatologic or metabolic diseases.
  • Active allergy within 4 weeks of the study
  • Symptoms of infection or inflammation within 4 weeks of the study
  • Pregnancy
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Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
EsmokeElectronic cigaretteInhaling e-cigarette vapor
Primary Outcome Measures
NameTimeMethod
Change in number of endothelial progenitor cells24 hours
Change in number of micro vesicles24 hours
Secondary Outcome Measures
NameTimeMethod
Change in exhaled nitric oxide24 hours

Trial Locations

Locations (1)

Institutionen för kliniska vetenskaper, Danderyds sjukhus

🇸🇪

Danderyd, Stockholm, Sweden

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