SAMSAM

Recruiting

Conditions: Respiratory tract infections

Registration Number: NL-OMON21999

Lead Sponsor: eiden University Medical Center

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Recruiting

Sex: Not specified

Target Recruitment: 45

Inclusion Criteria

a) Child aged 1-5 years of age attending day care, peuterspeelzaal or school at least 2 (half) days a week.
b) Parents ability and willingness to adhere to protocol-specified procedures, including availability of a freezer at home to store samples. This does not include donation of saliva by parents themselves, which is related to a secondary endpoint.
c) Written informed consent will be obtained from all legal representatives, for example both parents.

Exclusion Criteria

a) History of respiratory tract infections requiring hospitalization.
b) Current use of antibiotics, or antibiotics use in past four weeks.
c) Use of immune-altering medication (such as steroids, including inhaled corticosteroid).
d) History of severe concomitant disease (severe congenital heart disease, bronchopulmonary dysplasia, prematurity <32 weeks, cystic fibrosis, sickle cell disease, congenital or acquired immunodeficiency disorders, cardiovascular disease, neuromuscular disorders, oncology patients or major congenital anomalies).

Study & Design

Study Type: Observational non invasive

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Assess dynamics of pneumococcal colonisation using daily nasal sampling and the association of this with levels of pre-existing polysaccharide specific memory B cells.

Secondary Outcome Measures

Name	Time	Method
a) To validate the use of synthetic absorptive matrices (SAM) for detection of other respiratory pathogens versus NPS and saliva. <br>b) To assess dynamics of upper respiratory tract (URT) infection/colonisation for other pathogens. <br>c) To examine the relationship between local and systemic immune responses measured by SAM and URT infection/colonisation dynamics. <br>d) To define effect of incoming bacteria/viruses on microbiota and vice versa. <br>e) To measure host and microbial parameters associated with local immune boosting during colonisation/infection. <br>f) Associate common cold symptoms with URT colonisation/infection and related host responses. <br>g) Measure transmission between children and parents and immune responses in parents.