Depressed AIRE Gene Expression Causes Immune Cell Dysfunction & Autoimmunity in Down Syndrome

Not Applicable

Completed

• Conditions: Respiratory Tract Infections; Down Syndrome; Polyendocrinopathies, Autoimmune; Autoimmunity
• Interventions: Other: Phlebotomy

• Registration Number: NCT02421276
• Lead Sponsor: University of Colorado, Denver

Brief Summary

This study plans to learn more about Down syndrome. The investigators think there is a different level of the AIRE gene in individuals with Down syndrome. The investigators think that the AIRE gene level can provide more insight about depressed immune cell function in individuals with Down syndrome. Patients are being asked to be in this research study because the investigators want to see if their blood contains more of less of the AIRE gene.

Detailed Description

Down Syndrome (DS) is the most common chromosomal abnormality among live-born infants. Through full or partial trisomy of chromosome 21, DS is associated with cognitive impairment, congenital malformations (particularly cardiovascular), and dysmorphic features. In addition, immunological abnormalities are much more prevalent in individuals with DS. For example, DS is associated with increased susceptibility to infection, as revealed in 2009 during the influenza pandemic where the likelihood of death was 300 times greater for DS patients than the general population. DS patients have increased frequencies of autoimmune disorders and leukemias, yet curiously, have a decreased risk for allergic diseases, particularly asthma. Perhaps the most telling statistic for immunologic abnormality in DS patients is that respiratory tract infections are the most important cause of mortality in DS at all ages.Our studies have identified AIRE as a master control gene that is aberrantly decreased in persons with DS, leading to autoimmunity and immunologic abnormalities. AIRE ("autoimmune regulator"), although encoded on chromosome 21, is also significantly reduced in expression in DS, where it may contribute to autoimmune and immune dysregulation. The investigators will test the hypothesis that immune dysfunction and autoimmune disease preferentially occur in DS as a consequence of deficient expression of AIRE in peripheral blood cells.

Study Timeline

• Study First Submitted: 2015-04-15
• Study First Submitted QC: 2015-04-17
• Study First Posted: 2015-04-20
• Study Start: 2015-10-19
• Primary Completion: 2018-02
• Study Completion: 2018-02
• Status Verified: 2022-04
• Last Update Submitted: 2022-04-15
• Last Update Posted: 2022-04-19

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 45

Inclusion Criteria

1. Age newborn up until the twenty-second birthday.
2. Diagnosed with idiopathic or secondary pulmonary arterial hypertension as defined by a mean pulmonary artery pressure > 25 mmHg at rest or > 30 mmHg with exercise.
3. Confirmed trisomy 21.
4. Followed by the Pulmonary Hypertension Program and Sie Center at The Children's Hospital.
5. The investigator or co-investigator must obtain written informed consent and assent where applicable before any study procedure is performed or data is collected.

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Exclusion Criteria

1. Any person older than 22 years of age
2. Patients with sickle cell disease with Pulmonary Arterial Hypertension (PAH) as treatment is defined differently within this population.
3. In the opinion of the investigator, a patient who is unlikely to cooperate or complete the study for any reason.

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Persons with Down syndrome	Phlebotomy	White blood cell analysis from persons with Down syndrome assessed by presence of trisomy 21.
Persons without Down syndrome	Phlebotomy	White blood cell analysis from persons without Down syndrome assessed by absence of trisomy 21.

Primary Outcome Measures

Name	Time	Method
AIRE Gene expression in Macrophage Subpopulations	At the time of sample acquisition	Peripheral blood draw

Secondary Outcome Measures

Name	Time	Method
White blood cell Subpopulation Numbers	At the time of sample acquisition	Peripheral blood draw

Trial Locations

Locations (1)

University of Colorado, Denver; 🇺🇸 Denver, Colorado, United States