Study of the Expression of Heat Shock Proteins in Populations of B Lymphocytes in Human Tonsils

Not Applicable

Completed

• Conditions: Tonsillectomy
• Interventions: Procedure: tonsillectomy; Biological: blood sample

• Registration Number: NCT02859090
• Lead Sponsor: Centre Hospitalier Universitaire Dijon

Brief Summary

It has recently been shown that various HSPs are strongly expressed in B lymphomas. Indeed, HSP90 stabilizes the protein Bcl-6 in diffuse large-cell B lymphomas. Pharmacological inhibition of HSP90 in vitro induced apoptosis in lymphoma cells (Cerchietti et al. Nat. Medicine, 2009, 1369-1376). In addition, HSP110 is strongly expressed in non-Hodgkin lymphomas, and targeting this HSP at the cell surface using specific antibodies could constitute a future therapy (Zappasodi et al. Blood, 2011, 4421-4430). Despite the importance of these proteins in the development of lymphomas, their expression and their role in the activation of normal B lymphocytes and the normal development of germinal centres is not known.

The expression of Heat shock proteins should vary among the different B lymphocyte populations present in the tonsils (naïve B cells, memory B cells, germinal centre B cells).

The aim of this study is to establish an expression profile for heat shock proteins in populations of B lymphocytes in human tonsils

Detailed Description

Not available

Study Timeline

• Study Start: 2014-08-26
• Primary Completion: 2014-11-20
• Study Completion: 2014-11-20
• Study First Submitted: 2016-08-04
• Study First Submitted QC: 2016-08-04
• Study First Posted: 2016-08-08
• Status Verified: 2019-01
• Last Update Submitted: 2019-01-09
• Last Update Posted: 2019-01-10

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 5

Inclusion Criteria

• Persons who have provided oral consent
• Tonsillectomy in children or adults

Exclusion Criteria

• Persons without national health insurance cover
• Adults under guardianship
• Pregnant or breast-feeding women
• Persons with positive HIV or HCV serology

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
patient	tonsillectomy	-
patient	blood sample	-

Primary Outcome Measures

Name	Time	Method
expression of heat shock proteins	Day one

Trial Locations

Locations (1)

CHU Dijon Bourgogne; 🇫🇷 Dijon, France