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Evaluation of the Immunogenicity and Safety BBIBP-CorV Vaccine for COVID-19 in Adults in Guinea

Phase 2
Active, not recruiting
Conditions
Vaccine Adverse Reaction
COVID-19
Sars-CoV-2 Infection
Healthy Volunteer
Interventions
Biological: BBIBP-CorV
Registration Number
NCT05409300
Lead Sponsor
ANRS, Emerging Infectious Diseases
Brief Summary

Phase II, non-randomized, open-label, comparative, single center national trial in Guinea, aimed to assess the humoral vaccine immune response induced by BBIBP-CorV vaccine in 200 adults aged between 18 and 45 years or 55 or older, one month after receiving the complete COVID-19 vaccination schedule.

Detailed Description

The main objective of this phase II trial is to evaluate the humoral immune response induced by the BBIBP-CorV vaccine in adults one month after complete vaccination regimen against SARS-CoV-2, comparing younger (up to 45 years old) and elderly (55+ years old) populations.

200 participants will be included. The age categories are 18 - 45 years, 55 - 64 years and 65+ years. The number of participants per age group will be distributed as follows 1:1:0,5. There will be no comparison between population on different vaccines.

Participants will receive two doses of BBIBP-CorV at 28 days interval. Vaccines will be administered intramuscular (0.5mL per injection)

Humoral vaccine immune responses, induced by BBIBP-CorV will be measured by ELISA at D0, M1, M2, M6, M12 and M24.

Recruitment & Eligibility

Status
ACTIVE_NOT_RECRUITING
Sex
All
Target Recruitment
200
Inclusion Criteria
  • Age between 18 and 45 years old or 55 years and older
  • Be eligible to receive one of the study vaccines as part of the trial
  • Understand and agree to comply with study procedures (visits, telephone calls)
  • Agree not to participate in any other vaccine study during the time of the study
  • Give written informed consent prior to any examination performed as part of the trial
Exclusion Criteria
  • Age between 46 and 54 years old
  • Positive SARS-CoV-2 antigenic test
  • Positive SARS-CoV-2 PCR results less than 48 hours old
  • History of infection by COVID-19 confirmed within 3 months prior to inclusion
  • Symptoms compatible with COVID-19: sick or febrile participants (body temperature ≥ 38.0°C)
  • Pregnant or breastfeeding woman
  • Known chronic disease impacting the participant's immune response (uncured cancer, human immunodeficiency virus (HIV), hepatitis B virus (HBV) or hepatitis C virus (HCV) infection)
  • Anti-coagulant treatment
  • Immunosuppressive treatment
  • Contraindication to the proposed vaccine (according to RCP)
  • Patient having received immunoglobulin or another blood product within 3 months prior to inclusion
  • Previously received at least one injection of a SARS-CoV-2 vaccine
  • A history of serious adverse vaccine reactions (anaphylaxis and associated symptoms such as rash, breathing difficulties, laryngeal edema, or a history of allergic reaction that may be exacerbated by a component of the SARS-CoV-2 vaccine)
  • Any condition that, in the opinion of the investigator, may adversely affect the well-being of the participant and interfere with the purpose of the study

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
BBIBP-CorVBBIBP-CorVIntramuscular administration of two doses of vaccine at 28 days (+/- 2 days) interval (0.5 mL/dose)
Primary Outcome Measures
NameTimeMethod
Anti-SARS-CoV-2 Spike IgG level1 month after complete vaccination schema

Anti-SARS-CoV-2 Spike immunoglobulin G (IgG) level is measured using ELISA test

Secondary Outcome Measures
NameTimeMethod
Anti-SARS-CoV-2 IgM levelDay 0, Month 1, Month 2, Month 6, Month 12, and Month 24

Anti-SARS-CoV-2 immunoglobulin M (IgM) level is measured using ELISA test

Epitope profileDay 0, Month 2

Determination of epitope profile

Measurement of ultrasensitive IgA and mucosal IgA and IgM functionalityDay 0, Month 1, Month 2, Month 6, Month 12, and Month 24

Measurement of ultrasensitive IgA in saliva by Photoring assay Measurement of mucosal IgA and IgM functionality by SARS-CoV-2 mucosal IgA- and IgM-specific antibody-dependent cell-mediated cytotoxicity (ADCC) test

Rate of adverse eventsMonth 1 until Month 24

Rate of adverse events of any grade attributable to the vaccine or vaccination occurring between Month 1 and Month 24

Anti-SARS-CoV-2 IgA and levelDay 0, Month 1, Month 2, Month 6, Month 12, and Month 24

Anti-SARS-CoV-2 immunoglobulin A (IgA) level is measured using ELISA test

Neutralizing antibody level for SARS-CoV-2Day 0, Month 1, Month 2, Month 6, Month 12, and Month 24

Neutralizing antibody levels specific for SARS-CoV-2 and its variants (conventional in vitro neutralization and pseudo-neutralization assays)

Fluorospot tests (TH1, TH2, TH17, Cytotoxicity)Day 0, Month 2, Month 6

Fluorospot tests (Type 1,2,17 helper T cell (TH1, TH2, TH17), Cytotoxicity) Phenotyping of antigen-specific T cells by mass cytometry at Day 0 and Month 6 selected from the results of the Fluorospot test.

Anti-SARS-CoV-2 specific IgG levelDay 0, Month 1, Month 2, Month 6, Month 12, and Month 24

Anti-SARS-CoV-2 specific IgG level is measured using ELISA test

Mucosal SARS-CoV-2 specific antibody levelsDay 0, Month 1, Month 2, Month 6, Month 12

Mucosal SARS-CoV-2 specific antibody levels by measuring IgA, IgM and IgG in saliva using specific ELISAs

B cell response to vaccineDay 0, Month 2, Month 6

Determination of repertoire of B cells (stereotype clonotype)

SARS-CoV-2 infectionDay 0 until Month 24

Occurrence of confirmed COVID-19 cases during participant follow-up

Trial Locations

Locations (2)

Centre de recherche de Landreah

🇬🇳

Conakry, Guinea

Palais du Peuple

🇬🇳

Conakry, Guinea

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