NCT03717948
Completed
Not Applicable
Examining the Impact of Acute Blood Flow Restricted Aerobic Exercise on PGC-1α mRNA Expression in Young Healthy Males: A Randomized Cross-over Trial
ConditionsMitochondrial Biogenesis
Overview
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- Queen's University
- Enrollment
- 13
- Locations
- 1
- Primary Endpoint
- Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA)
Overview
Brief Summary
This crossover study investigates the effects of restricting arterial blood flow during cycling exercise on PGC-1α and its upstream signalling pathways. In a randomized order, participants will exercise once for 30 minutes with restricted blood flow and then will exercise once for 30 minutes with no blood flow restriction.
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Crossover
- Primary Purpose
- Basic Science
- Masking
- Double (Investigator, Outcomes Assessor)
Masking Description
Allocation concealment, assessor blinding
Eligibility Criteria
- Ages
- 18 Years to 30 Years (Adult)
- Sex
- Male
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •3 hours of physical activity per week
- •No previous cycling training
- •No concurrent involvement in another training program
- •Body mass index \< 30 kg/m2
Exclusion Criteria
- •Presence of cardiovascular disease
- •Presence of metabolic disease
- •Taking regular oral medication
- •Current smoker
Outcomes
Primary Outcomes
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA)
Time Frame: 15 minutes before exercise and 3 hours post-exercise for both exercise sessions
Fold-change in PGC-1α mRNA expression
Secondary Outcomes
- Catecholamine concentrations(15 minutes before exercise and immediately post-exercise for both exercise sessions)
- Total and phosphorylated acetyl coenzyme A carboxylase (ACC)(15 minutes before exercise and immediately post-exercise for both exercise sessions)
- Total and phosphorylated protein kinase A (PKA)(15 minutes before exercise and immediately post-exercise for both exercise sessions)
- Heart rate (HR)(During both exercise sessions)
- Total and phosphorylated p38 mitogen-activated protein kinase (MAPK)(15 minutes before exercise and immediately post-exercise for both exercise sessions)
- Blood lactate(During both exercise sessions (lactate); 15 minutes before and immediately after both exercise sessions)
- Oxygenated and deoxygenated hemoglobin(During both exercise sessions and up to 10 minutes after (for cuff occlusion))
- Acetylated p53(15 minutes before exercise and immediately post-exercise for both exercise sessions)
- Revolutions per minute (RPM)(During both exercise sessions)
- Ratings of perceived exertion (RPE)(During both exercise sessions)
Investigators
Brendon Gurd
Associate Professor
Queen's University
Study Sites (1)
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