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Filling Key Research Gaps With Clinical Implications in Mansonellosis and Schistosomiasis: a Network Approach

Not Applicable
Recruiting
Conditions
Schistosoma Mansoni
Interventions
Diagnostic Test: Molecular biology analyses
Registration Number
NCT06311344
Lead Sponsor
IRCCS Sacro Cuore Don Calabria di Negrar
Brief Summary

This is an experimental, multicenter, non-profit study promoted by the Department of Infectious and Tropical Diseases of the IRCCS (Istituto di Ricerca e Cura a carattere scientifico) Sacro Cuore Don Calabria of Negrar which aims to evaluate the presence of endosymbiont Wolbachia in circulating microfilariae obtained from biological samples of individuals infected with M .perstans and the presence of infection with hybrid Schistosoma species in the population of Schistosoma eggs isolated from biological samples of patients with active schistosomiasis.

Detailed Description

Not available

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
200
Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Biological samplesMolecular biology analysesM. perstans microfilariae and Schistosoma spp eggs, obtained from the routine diagnostic procedures carried out on patients, specifically migrants and travellers, visited in the centres participating to this study
Primary Outcome Measures
NameTimeMethod
Percentage of Schistosoma eggsBaseline

Percentage of samples defined as the whole Schistosoma eggs retrieved from one stool or urine sample from each patient, positive for the presence Schistosoma hybrid species.

Percentage of M. perstans microfilariaeBaseline

Percentage of samples, defined as the whole M. perstans microfilariae retrieved from one blood sample from each patient, positive for the presence of Wolbachia as assessed by PCR.

Number of Schistosoma eggsBaseline

Number of samples defined as the whole Schistosoma eggs retrieved from one stool or urine sample from each patient, positive for the presence Schistosoma hybrid species.

Number of M. perstans microfilariaeBaseline

Number of samples, defined as the whole M. perstans microfilariae retrieved from one blood sample from each patient, positive for the presence of Wolbachia as assessed by PCR.

Secondary Outcome Measures
NameTimeMethod
Identification of hybrid species of Schistosoma spp.Baseline

Identification of hybrid species from each parental species of Schistosoma spp.

Percentage of samples positive for WolbachiaBaseline

Percentage of samples, defined as the whole M. perstans microfilariae retrieved from one blood sample from each patient, positive for the presence of Wolbachia as assessed by PCR, by country of origin of the patient or most likely country of infection.

Number of samples positive for WolbachiaBaseline

number of samples, defined as the whole M. perstans microfilariae retrieved from one blood sample from each patient, positive for the presence of Wolbachia as assessed by PCR, by country of origin of the patient or most likely country of infection.

Number of samples positive for Schistosoma hybrid speciesBaseline

number of samples defined as the whole Schistosoma eggs retrieved from one stool or urine sample from each patient, positive for the presence Schistosoma hybrid species by country of origin of the patient or most likely country of infection.

Percentage of samples positive for Schistosoma hybrid speciesBaseline

percentage of samples defined as the whole Schistosoma eggs retrieved from one stool or urine sample from each patient, positive for the presence Schistosoma hybrid species by country of origin of the patient or most likely country of infection.

Trial Locations

Locations (1)

IRCCS Sacro Cuore Don Calabria hospital

🇮🇹

Negrar, Verona, Italy

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