Effect of Antigens or Therapeutic Agents on in Vitro Human Intestinal Organoids
Active, not recruiting
- Conditions
- Intestine Disease
- Registration Number
- NCT03256266
- Lead Sponsor
- University of Erlangen-Nürnberg Medical School
- Brief Summary
The study evaluates the effect of nutrient antigens or therapeutic agents on human small intestinal organoids.
- Detailed Description
Small intestinal biopsies will be taken to establish human intestinal organoids in vitro. The organoids will be propagated and various antigens will be checked for their effects on proliferation, production of inflammatory components or signal cascade.
Recruitment & Eligibility
- Status
- ACTIVE_NOT_RECRUITING
- Sex
- All
- Target Recruitment
- 375
Inclusion Criteria
- patients with gastrointestinal disorders and therapeutical indication of gastroduodenoscopy or coloscopy
- healthy controls for preventive medical check-up
Exclusion Criteria
- pregnant women
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Determination of proliferation, apoptosis, histology, and cytokine Expression before and after Antigen provocation of organoids with gluten peptides, nutritional Antigens, Glucagon like peptide antagonist, or Tumor nekrose factor antagonists baseline vs 24h vs 48h vs 72h Determination of effects on organoids after antigen Stimulation with ELISA, histochemistry, RNA or proteome analysis
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
Department of Medicine 1, Hector Center for Nutrition, Exercise and Sports, Friedrich-Alexander-University Erlangen-Nuremberg
🇩🇪Erlangen, Germany