A New Micro Swim-up Procedure for Sperm Preparation in ICSI Treatment
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 100
- 试验地点
- 2
- 主要终点
- microbiological test
研究概览
简要总结
The aim of this study is to evaluate the microbiological safety and the efficacy of a new not expensive, easy and fast technique (Direct Microswimup) for sperm preparation in In Vitro Fertilization (IVF) treatments without the use of centrifuge, able to simplify the procedure and to avoid mismatches by ensuring the same results of the standard techniques in terms of fertilization rate, blastulation rate, pregnancy/abortion rate, cryostorage rate, and a better euploid status of the embryos.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Retrospective
入排标准
- 年龄范围
- 20 Years 至 35 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •at least 8 oocytes retrieved at pickup
- •oocyte insemination with ICSI technique
- •only elective single embryo transfer (eSET) at blastocyst stage
- •sperm concentration ≥ 1 X 106 /ml
- •absence of antibiotic treatment in the previous four weeks for male patients
排除标准
- •recurrent miscarriages
- •more than three in vitro fertilization treatment
- •azoospermia
结局指标
主要结局
microbiological test
时间窗: 3 months
A standard bacteriological culture \[based on inoculation of blood and "chocolate" agar plates and Brain-Heart Infusion broth, followed by incubation at 37°C for up to 72 hours\] was performed in order to investigate the presence and amount of colonizing bacteria delivered with the semen at ejaculation and after treatment
次要结局
- semen treatment efficacy(6 months)
研究者
Palini Simone
Embryologist
Cervesi Hospital, Cattolica, Italy