Can incubating sperm in a new sperm washing media 'SpermFAST', prior to in vitro fertilization (IVF) result in fertilization.

Not Applicable

Terminated

• Conditions: infertility; sub fertility; Reproductive Health and Childbirth - Fertility including in vitro fertilisation

• Registration Number: ACTRN12619000370101
• Lead Sponsor: niversity of Adelaide

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Study Start: 2019-03-08
• Study Completion: 2023-06-30
• Last Update Posted: 26 September 2023

Recruitment & Eligibility

• Status: Stopped early
• Sex: All
• Target Recruitment: 4

Inclusion Criteria

Any couple undergoing standard IVF insemination for assisted reproductive technology treatment.
- Able to read and speak English.

Exclusion Criteria

Withdrawal from study;
- The quality of the washed sperm does not hit the recommended values for IVF insemination (at least 1x106/ml with 90% motility) - these patients will automatically be transferred to ICSI insemination.
- Less than 6 eggs collected at egg pick up. Data suggests that less than 6 eggs collected at egg pick up is associated with increased rates of failed fertilization.

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Failed Fertilisation<br>~16h post insemination embryo laboratory staff will assess the egg for signs of fertilization by for the presences of 2 pro-nuclei. Eggs without 2 clearly defined pro-nuclei will be deemed not fertilized. [ 16-20 hrs post co incubation of sperm and eggs]

Secondary Outcome Measures

Name	Time	Method
Fertilisation rate (%)<br>~16h post insemination embryo laboratory staff will assess the egg for signs of fertilization by for the presences of 2 pro-nuclei. Eggs with 2 clearly defined pro-nuclei will be deemed fertilized. Fertilization rate will be calculated by the number of fertilized eggs to the total number of eggs inseminated. [ 16-20 hrs post co incubation of sperm and eggs]