Acute Effect of „electronic – cigarette-use on vascular endothelial functio
Not Applicable
- Conditions
- healthy volunteersnicotine dependence
- Registration Number
- DRKS00007930
- Lead Sponsor
- niversitätsmedizin Mainz, II. Medizinische Klinik
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Complete
- Sex
- All
- Target Recruitment
- 50
Inclusion Criteria
Non-smokers and smokers age 18-60
Exclusion Criteria
•Smoking of tobacco products within the preceding 6 months (only cohort 1)
•Current use of long acting nitrates or calcium antagonists
•History of asthma or chronic pulmonary disease
•Vascular or other abnormalities precluding application of a pressure cuff on the arm to conduct FMD/FMC measurement
•symptomatic cardiovascular disease
Study & Design
- Study Type
- interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Endothelial function (flow-mediated dialtion FMD % of A. brachialis) as change before and after vapor exposition measured with high resolution ultrasound before and after cuff occlusion as described in guidelines.
- Secondary Outcome Measures
Name Time Method FMC (flow mediated contraction as described by Gori measured by ultrasound corresponding to FMD) measured before and after vapor exposure;<br>blood pressure measured with Riva-Roci and contiously before , during and after vapor exposure using pulse transit time<br>heart rate (ECG derived, holter monitoring before and during exposure)<br>sympathic nerve activity (laser Doppler before and after exposure)<br>sympathovagal Balance (derived from ECG heart variability measured with holter ECG during study period)<br>oxdiative Stress (blood)<br>Effect of antioxidative substances on FMD (measured as effect of p.o. VitC application on FMD after exposure)<br>Endothelium activation (measured in blood before and after vapor from frozen samples with the use of ELISA kits (E-selectine, VCAM etc.))<br>Oxidative stress (measured from frozen blood samples befor and after exposure with ELISA kits TBARS, MDA, OxLDL, MPO)<br>