Epithelial Ion Transport Defects in Pancreatitis: establishment of an organoid model

Recruiting

• Conditions: chronic pancreatitis; inflammation of the pancreas; 10015674

• Registration Number: NL-OMON52348
• Lead Sponsor: Erasmus MC, Universitair Medisch Centrum Rotterdam

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Results First Posted: 1900-01-01
• Last Update Posted: 9 April 2024

Recruitment & Eligibility

• Status: Recruiting
• Sex: Not specified
• Target Recruitment: 9

Inclusion Criteria

A subject must meet all of the following criteria:
- Must be an adult (>18 years old)
- Must be undergoing pancreatoscopy within the framework of regular diagnostics
or treatment in the Erasmus MC
- Must sign informed written consent

Exclusion Criteria

None

Study & Design

• Study Type: Observational invasive
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>Assess the ability to culture PDEC organoids from pancreatic duct<br /><br>microbiopsies, to study the pathophysiology and pharmacotherapy of pancreatitis<br /><br>in vitro.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>• Asses the phenotype of PDEC organoids derived from pancreatitis patients, as<br /><br>compared to those derived from non-pancreatitis patients, by investigation of:<br /><br>o Genetic makeup<br /><br>o Gene and protein expression<br /><br>o Ion transport<br /><br>o Response to known pancreatitis-inducers and/or ion transport modulators, to<br /><br>further elucidate the molecular mechanisms involved in the disruption of CFTR<br /><br>function in PDECs in pancreatitis and potentially to advance the development of<br /><br>pharmacotherapy for pancreatitis.<br /><br>• Assess the ability of duodenal organoids to mimic key features of ion<br /><br>transport in PDEC organoids, to explore a more readily accessible surrogate<br /><br>model to study the pathophysiology and pharmacotherapy of pancreatitis in vitro.<br /><br></p><br>