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Effectiveness of PRP, Conditioned Medium UC-MSCs Secretome and Hyaluronic Acid for the Treatment of Knee Osteoarthritis

Phase 1
Completed
Conditions
Knee Osteoarthritis
Interventions
Biological: Platelet-rich Plasma
Biological: Conditioned Medium From Umbilical Cord Mesenchymal Stem Cell Culture (MSCs) Secretome
Drug: Low Molecular Weight Hyaluronic Acid
Registration Number
NCT05579665
Lead Sponsor
Universitas Sriwijaya
Brief Summary

The clinical trial will be carried out at the Dr. Moh. Hoesin Central Hospital, Palembang and planned from October 2022 to March 2023.

This study aims to compare the efficacy of Platelet Rich Plasma, Conditioned Medium From Umbilical Cord Mesenchymal Stem Cell (MSC) Culture Secretome and hyaluronic acid for the Treatment of Knee Osteoarthritis

Detailed Description

In this, prospective, Open-label study, patients with mild to moderate symptomatic knee osteoarthritis will be randomized to receive either a series of platelet-rich plasma or hyaluronic acid or Conditioned Medium From Umbilical Cord Mesenchymal Stem Cell (MSC) Culture Secretome injections under ultrasound guidance. Each of the groups were given 5 times injection 3 cc platelet-rich plasma or 2 cc hyaluronic acid or 2cc Conditioned Medium From Umbilical Cord MSC Secretome at intervals 1 weeks.

Clinical data in the form of subjective outcome measures will be collected pre-treatment and 6 months after injection.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
45
Inclusion Criteria
  • Patients aged 30-60 years
  • Suffering from grade 2 and 3 osteoarthritis (OA) was identified by two observers who differed accordingly the Kellgren and Lawrence research scale
  • Patient with knee pain that had been continuing for at least 12 months with no relief using anti-inflammatory medications and that deteriorated with weight-bearing
  • Patients can understand the nature of the study and written informed consent is given to patients
Exclusion Criteria
  • Age > 60 years
  • Acute Knee Osteoarthritis Effusions
  • Patients are not willing to obey the study protocol
  • There are signs of infection local or general infection or positive serology for HIV, hepatitis and syphilis
  • There is a congenital disease that causes significant deformity of the knee can interfere with cell applications and interpret results
  • Articular injection of the knee by any drug during the previous 3 months
  • Participate in any clinical trial or treatment 30 days before the study
  • Other conditions may, according to medical criteria, not support participation in this research (The recent history of knee trauma, Autoimmune rheumatic diseases, Uncontrolled systemic diseases such as diabetes or hypertension, patient with Immunosuppressive or anticoagulant treatment and cancer)

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Platelet-rich Plasma (PRP)Platelet-rich PlasmaPlatelet-rich plasma administered 5 times as an intra-articular injection under ultrasound guidance as a series of one weekly injections to the affected knee. 1 weekly injections are of leukocyte poor, buffer/additive free, singe spin, platelet-rich plasma averaging 3mL in volume.
Conditioned Medium From Umbilical Cord Mesenchymal Stem Cell Culture (MSCs) SecretomeConditioned Medium From Umbilical Cord Mesenchymal Stem Cell Culture (MSCs) SecretomeConditioned Medium From Umbilical Cord Mesenchymal Stem Cell Culture (MSCs) Secretome administered 5 times as an intra-articular injection under ultrasound guidance as a series of one weekly injections to the affected knee. 1 weekly injections are Conditioned Medium From Umbilical Cord Mesenchymal Stem Cell Culture (MSCs) Secretome averaging 2 mL in volume.
Hyaluronic Acid (HA)Low Molecular Weight Hyaluronic AcidHyaluronic Acid administered 5 times as an intra-articular injection under ultrasound guidance as a series of one weekly injections to the affected knee. 1 weekly injections are of low molecular weight hyaluronan in a 2mL injection.
Primary Outcome Measures
NameTimeMethod
Visual Analog Scale (VAS)6 month after injection

To asses pain score, score 1(good)-10(worst)

Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC)6 month after injection

To evaluate the condition of patients with osteoarthritis, including pain, stiffness, and physical functioning of the joints. The WOMAC is a self administered questionnaire consisting of 24 items divided into subscales including pain (0-20), stiffness (0-8) and physical function (0-68). Higher scores indicate worse pain, stiffness and functional limitations.

Secondary Outcome Measures
NameTimeMethod
Laboratory Assessment3 month after injection

Cartilage Oligomeric Matrix Protein (COMP)

Trial Locations

Locations (1)

Mohammad Hoesin Central General HospitalPalembang

🇮🇩

Palembang, South Sumatera, Indonesia

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