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Beetroot Juice Effects on Contractile or Neuromuscular Properties in Male Trained Sprinters

Not Applicable
Completed
Conditions
Nitrates
Placebo
Registration Number
NCT06675682
Lead Sponsor
Universidad Pontificia Comillas
Brief Summary

Beetroot juice is a rich source of nitrate (NO3-), which serves as a precursor to nitric oxide (NO) via the NO3- to nitrite (NO2-) to NO conversion pathway. This pathway is believed to enhance vasodilation and improve neuromuscular function, such as by increasing sarcoplasmic reticulum calcium release and re-uptake, leading to enhanced force production in type II muscle fibers and improved muscle contractile recruitment. Therefore, the aim of this study was to investigate the effects of beetroot juice supplementation on muscle contractile properties, as assessed by tensiomyography, and neuromuscular performance in sprint-trained athletes.

Detailed Description

Sports nutrition is a rapidly expanding scientific field that has experienced a significant increase in interest from the academic community over the past decade. Despite the extensive marketing of numerous nutritional products that claim to optimize athlete health, function, and performance, only a limited number of sports foods or dietary supplements have been supported by robust evidence for enhancing performance. Among these, caffeine, creatine, beetroot juice, β-alanine, and bicarbonate are notable for their validated efficacy, though the effectiveness of these supplements can vary based on the specific event, context of use, and the individual athlete's goals and responsiveness.

Beetroot juice, in particular, is a rich source of nitrate (NO3-), which serves as a precursor to nitric oxide (NO) through the NO3- to nitrite (NO2-) to NO conversion pathway. This pathway is believed to promote vasodilation and improve neuromuscular function, such as by increasing sarcoplasmic reticulum calcium release and re-uptake, leading to enhanced force output in type II muscle fibers and improved muscle contractile recruitment. Therefore, the aim of this study was to investigate the effects of beetroot juice supplementation on muscle contractile properties, as assessed by tensiomyography, and neuromuscular performance in sprint-trained athletes. The goal was to gain a deeper understanding of the physiological effects induced by beetroot juice intake in short-distance track and field disciplines.

Recruitment & Eligibility

Status
COMPLETED
Sex
Male
Target Recruitment
12
Inclusion Criteria
  • Between 18 to 40 years old.
  • With a regular training practise in track and field disciplines > 5 years.
Exclusion Criteria
  • Intolerance to beetroot juice or NO3- derivatives
  • Presence of any chronic pathology or injury within three months prior to the study-
  • Use of medications or supplements (e.g., caffeine) during the study.
  • Failure to attend all experimental sessions.
  • Failure to adhere to the dietary guidelines established in the study

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Primary Outcome Measures
NameTimeMethod
Tensiomyography changes (seconds): Delay time (Td) ; Contraction time (Tc) ; Sustain time (Ts) ; Relaxation time (Tr)1-week

The measurement of the rectus femoris, biceps femoris, gastrocnemius lateralis, and gastrocnemius medialis muscles was conducted using tensiomyography. We analyzed different parameters such as selay time (Td) as a time between the electrical impulse and 10% of the contraction; Contraction time (Tc) as a time between 10% and 90% of the contraction; Sustain time (Ts) as a time between 50% of the contraction and 50% of the relaxation; Relaxation time (Tr) as a time between 90% and 50% of the relaxation.

Changes in 60-m and 100-m sprint test (seconds)1-week

Time to complete 60-m and 100-m sprint test using photocell timing gates

Secondary Outcome Measures
NameTimeMethod
Changes in rate of perception effort1-week

Using rate of perceived exertion scale (1-10 points)

Changes in maximal countermovement and squat jump height (cm)1-week.

Maximal jump height using a contact platform

Changes in nitrites and nitrates levels in saliva (μmol/L)1-week

Using specific kit ELISA analysis

Changes in side effects questionnarie1-week

Questionnarie based on side-effects related to beetroot juice ingestion (A scale consisting of nine items, each answered with "yes" or "no")

Trial Locations

Locations (1)

Universidad Pontificia Comillas

🇪🇸

Madrid, Spain

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