Glycemic Index of Mango and Pear in Different Solid Products and Drinks
- Conditions
- Glucose Intolerance
- Interventions
- Other: Mango puree and pear juice beverageOther: Commercial mango juiceOther: 50 grams glucose loadOther: Mix of natural mango and pearOther: Fresh natural mangoOther: Frozen natural mangoOther: Processed natural mango pulp
- Registration Number
- NCT01929551
- Lead Sponsor
- Instituto Tecnologico y de Estudios Superiores de Monterey
- Brief Summary
The purpose of this study is to compare the glycemic index of a beverage made of natural concentrated mango puree with natural concentrated pear juice with other five different products based on natural mango and pear ingredients in the form of fruit or juice against a control of 50-gram glucose load.
- Detailed Description
The participants will be recruited with an open invitation to participate, those who voluntarily accept, sign an informed consent, and meet the all inclusion criteria and none of the exclusion criteria will participate.
Each participant will receive a different product (of the seven products available) each day of the test (8 days in total), making sure everyone receives each one of the products. All products will be distributed in a randomized fashion for each of the participants. The 50-gram glucose load (gold standard) will be the reference or control, and will be taken twice by each subject in the test.
Each one of the products given will contain 50 grams of carbohydrate. The time given to consume the products will be 10 minutes total.
There will be a "wash out" period of three days in between each product.
The participants will eat the product and capillary glucose will be taken with a glucometer (Accu-Chek Performa System © 2009 Roche Diagnostics. Roche Products, México) at times: 0, 15, 30, 45, 60, 75, 90, 150 and 120 minutes. The glucometer will be calibrated with a standard glucose solution every day previous to the test. The results will be used to calculate the glycemic index. At the end of the 120 minutes, the participants will receive a questionnaire of digestive symptoms presented during the test.
The glycemic index will be calculated by the area under the curve for each product divided by the area under the curve for the 50-gram glucose load, multiplied by 100. The area under the curve used to calculate the glycemic index is the area that surpasses the basal line, excluding the area under the basal line according to the Food and Agriculture Organization (FAO) in 1998 (Brouns et al 2005).
All information collected will be emptied into a pre-designed database.
The differences between the different products ingested will be analyzed with the statistical test of analysis of variance (ANOVA) with a pre-test of homogeneity of variance Brown-Foryhte. In case of failure to find homogeneity in the variances the nonparametric ANOVA, the Kruskal-Wallis test, will be used. The results will be expressed as mean ± standard deviation for each with a significance level of 0.05 for all the statistical tests.
After ANOVA, Tuckey Multiple Comparisons test will be used to evaluate the pairwise relationship for each product.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 38
- Men and women
- Ages 18 to 50 years
- Body mass index between 18.5 and 27.4 kilograms divided by squared meters
- Waist circumference ≤ 102 centimeters for men and ≤88 centimeters for women
- Fasting capillary glucose ≤100 milligrams per deciliter
The night before the study:
- 10 hours fasting
- Light dinner of their choice and repeat it every time before the study
- No alcohol, coffee or tea drinks the previous night
- No vigorous exercise the day before the test nor the morning of the test
- Consumption of any of the following: oral hypoglycemic drugs, oral or parenteral glucocorticoid drugs, thyroid hormones, stimulant of intestinal motility or other drugs that may interfere with the intestinal absorption during the test
- Acute or chronic gastrointestinal infections
- Breast feeding or pregnant women
- Intestinal surgeries in the last 6 months
- Allergies to any of the products we will be using
- Subjects with gastrointestinal or metabolic diseases
- Subjects with first grade family history of diabetes mellitus
- Subjects with glucose intolerance, insulin resistance or diabetes mellitus
- Subjects who dislike any of the products we will be using
- Subjects with an acute (previous 24 hours) digestive crisis including: gastritis, diarrhea, and vomit, among others
- Subjects with a history of mal-absorption
Suspension:
- Subjects that do not arrive at the test-day
- Fasting glucose ≥ 100 milligrams per deciliter the day of the test
- Subjects who develop gastrointestinal disease during the study time frame
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- FACTORIAL
- Arm && Interventions
Group Intervention Description Mango puree and pear juice beverage Mango puree and pear juice beverage Participants will drink 450 milliliters of the juice at time 0. Commercial mango juice Commercial mango juice Participants will drink 450 milliliters of the juice at time 0. 50 grams glucose load 50 grams glucose load Participants will drink 250 milliliters of the standard glucose load at time 0. Mix of natural mango and pear Mix of natural mango and pear Participants will eat 400 to 500 grams of a mixture of fresh natural fruit containing mango (73%) and pear (27%), along with 250 milliliters of water at time 0. Fresh natural mango Fresh natural mango Participants will eat 400 to 500 grams of frozen mango pulp, along with 250 milliliters of water at time 0. Frozen natural mango Frozen natural mango Participants will eat 400 to 500 grams of frozen mango pulp, along with 250 milliliters of water at time 0. Processed natural mango pulp Processed natural mango pulp Participants will eat 400 to 500 grams of frozen mango pulp, along with 250 milliliters of water at time 0.
- Primary Outcome Measures
Name Time Method Glycemic index of mango puree with natural pear juice beverage 8 days Glycemic index will be calculated for 450 milliliters of this juice. The results will be expressed as mean ± standard deviation for each with a significance level of 0.05 for all the statistical tests.
- Secondary Outcome Measures
Name Time Method Glycemic index of a commercial mango juice 8 days Glycemic index will be calculated for 450 milliliters of this juice. The results will be expressed as mean ± standard deviation for each with a significance level of 0.05 for all the statistical tests.
Glycemic index of natural fresh mango 8 days Glycemic index will be calculated for 400 to 500 grams of mango along with 250 milliliters of water. The results will be expressed as mean ± standard deviation for each with a significance level of 0.05 for all the statistical tests.
Glycemic index of natural frozen mango pulp 8 days Glycemic index will be calculated for 400 to 500 grams of mango along with 250 milliliters of water. The results will be expressed as mean ± standard deviation for each with a significance level of 0.05 for all the statistical tests.
Glycemic index of processed natural mango pulp 8 days Glycemic index will be calculated for 400 to 500 grams of mango along with 250 milliliters of water. The results will be expressed as mean ± standard deviation for each with a significance level of 0.05 for all the statistical tests.
glycemic index of a mixture of fresh mango 73 percent and fresh pear 27 percent 8 days Glycemic index will be calculated for 400 to 500 grams of mango along with 250 milliliters of water. The results will be expressed as mean ± standard deviation for each with a significance level of 0.05 for all the statistical tests.
glycemic index of a 50 gram glucose load 8 days Glycemic index will be calculated for a standard 50-gram glucose load. This will be done twice by every participant. The results will be expressed as mean ± standard deviation for each with a significance level of 0.05 for all the statistical tests.
Trial Locations
- Locations (1)
Clinical Nutrition and Obesity Research Center. School of Medicine and Health Sciences, TEC Salud, Tecnológico de Monterrey
🇲🇽Monterrey, Nuevo Leon, Mexico