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Micro Glucagon During Exercise in Type 1 Diabetes

Early Phase 1
Withdrawn
Conditions
Type 1 Diabetes
Hypoglycemia
Interventions
Drug: Mini-glucagon
Drug: Micro-glucagon
Registration Number
NCT04192019
Lead Sponsor
Insel Gruppe AG, University Hospital Bern
Brief Summary

This proof-of-principle study to assess effects of different doses (mini and micro) of subcutaneous glucagon analog Dasiglucagon (Zealand Pharma, Copenhagen, Denmark) on the change in blood glucose concentration during moderate-intensity exercise in people with T1D.

Detailed Description

People with type 1 diabetes (T1D) are recommended to engage in regular exercise for a variety of health and fitness reasons. However, moderate-intensity exercise is associated with an increased risk of hypoglycaemia in people with T1D. Current guidelines are to reduce insulin dose and/or increase carbohydrate consumption in the context of the exercise bout. However, despite the many advances in insulin formulations and delivery devices, hypoglycaemia remains a significant risk. Mini-dose glucagon taken before an exercise bout has been shown to be an effective non-caloric strategy to prevent exercise-induced hypoglycaemia. However, even the reduced doses (150-200 µg) used in previous studies might still be rather high translating into potential side-effects (i.e. hyperglycaemia, gastrointestinal symptoms, etc.). Lower doses (below 100 µg, micro-glucagon) may be sufficiently effective to counteract hypoglycaemia risk associated with exercise and associated with better tolerance. Moreover, there is little information on the effects of subcutaneous glucagon on glycogen stores and changes in exercise metabolism. Greater understanding of exercise-associated metabolism following mini- and micro-dose glucagon using techniques such as magnetic resonance spectroscopy (MRS), continuous glucose monitoring (CGM), stable isotope tracers, and indirect calorimetry may result in novel approaches to improve blood glucose management in people with T1D. Utilising these techniques may also further our understanding of the optimal glucagon dosing (timing and amount) during exercise to manage hypoglycaemia and reduce the risk of adverse events. Proof-of-principle study to assess effects of different doses (mini and micro) of subcutaneous glucagon analog Dasiglucagon (Zealand Pharma, Copenhagen, Denmark) on the change in blood glucose concentration during moderate-intensity exercise in people with T1D. Secondly, to investigate exercise-metabolism following Dasiglucagon injection using 3 tesla magnetic resonance spectroscopy (MRS) and indirect calorimetry. Third, to assess participant experience of Dasiglucagon during exercise and the incidence of adverse events.

Recruitment & Eligibility

Status
WITHDRAWN
Sex
All
Target Recruitment
Not specified
Inclusion Criteria
  • T1D for >1 year
  • Male aged 18-45 years old
  • HbA1c <8.0% (64 mmol/mol) based on analysis from the central laboratory unit of Bern University Hospital
  • Regular physical activity (defined as meeting 150 min moderate-intensity exercise per week)
  • Using either continuous subcutaneous insulin infusion or multiple daily injections
  • Written informed consent
Exclusion Criteria
  • Physical or psychological disease likely to interfere with the normal conduct of the study and interpretation of the results as judged by the investigator
  • Current treatment with drugs known to interfere with metabolism e.g. systemic corticosteroids, statins, SGLT2 inhibitors, GLP1 agonists
  • Relevant diabetic complications as judged by the investigator
  • Body mass index 30 kg/m2
  • Uncontrolled hypertension (>180/100 mmHg)

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
Mini-dose glucagonMini-glucagon150 µg mini-dose of subcutaneous Dasiglucagon 5 min before exercise the start of exercise
Micro-dose glucagonMicro-glucagon80 µg (micro-dose) subcutaneous Dasiglucagon 5 min before the start of exercise
Primary Outcome Measures
NameTimeMethod
Time to hypoglycaemia60 minutes

Time (in minutes) to hypoglycaemia (plasma glucose \<3.9mmol/l) during 60 minutes of aerobic exercise in individuals with type 1 diabetes using mini and micro doses of Dasiglucagon compared to no Dasiglucagon

Secondary Outcome Measures
NameTimeMethod
Any adverse events24 hours

Adverse symptoms following glucagon/placebo use

Glucose during exercise60 minutes

Mean glucose concentration and area under the glucose curve during exercise

Hypoglycaemia24 hours

Incidence of hypoglycaemia (≤3.9 mmol/L for 15 min or more) during the 24 hour post exercise recovery period

Glucagon during exercise60 minutes

Mean glucagon concentration and area under the glucagon curve during exercise

Change in glycaemia during exercise60 minutes

Change in blood glucose concentration, calculated based on the participants' glucose at the start of exercise and the last value measured at the end of exercise. If the exercise is stopped early because of hypoglycaemia, the last exercise glucose value will be used for analysis

Time in target24 hours

Time in target glycaemic range (4-10 mmol/L) in the recovery period and overnight

Time in target during exercise60 minutes

Time in target glycaemic range (4-10 mmol/L) during exercise

Glycogen content4 hours

Change in skeletal muscle and hepatic glycogen following exercise

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