Sequential Versus Single O2 Levels on Human Embryo Development
Not Applicable
Completed
- Conditions
- Embryo Culture
- Interventions
- Other: Sequential O2 Culture
- Registration Number
- NCT03630900
- Lead Sponsor
- Ibn Sina Hospital
- Brief Summary
Myriads of elements affect human embryo culture in vitro. These include, but not limited to, air quality, culture media, culture temperature, incubator humidity, pH, and O2 levels. O2 tension has been thoroughly investigated and, to date, 5% O2 level is superior to 20%. Reports showed that lowering the O2 from Day 3 of embryo culture seems to be physiological.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Female
- Target Recruitment
- 487
Inclusion Criteria
- ICSI cycles undergoing their first ICSI attempt
Exclusion Criteria
- ICSI cycles with a previous failure
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Sequential O2 culture (5% then 2.5%) Sequential O2 Culture Embryo culture from day 0 to 3 in 5% O2 then split to either 5% or 2.5% O2 until Day 5 or 6.
- Primary Outcome Measures
Name Time Method Ongoing Implantation rate three months number of sacs with a heartbeat after week twelve of gestation
- Secondary Outcome Measures
Name Time Method clinical and ongoing pregnancies three months registered pregnancy with fetal heartbeat at week four after transfer and after week twelve of gestation
human embryo development in vitro three months
Trial Locations
- Locations (3)
Banon Assiut
🇪🇬Assiut, Egypt
Qena Fertility Center
🇪🇬Qena, Egypt
IbnSina IVF Center
🇪🇬Sohag, Egypt