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Evaluation of Coagulation Status of Pregnant Women in the Peripartum Period Using the Device Clotpro®

Completed
Conditions
Coagulation and Hemorrhagic Disorders
Interventions
Diagnostic Test: Coagulation test on device ClotPro
Registration Number
NCT05935137
Lead Sponsor
Ondrej Hrdy
Brief Summary

The primary aim of the study is to determine the physiological parameters of the coagulation status of pregnant women in the peripartum period (PWPP) for the ClotPro® device - Ex-test (CT, CFT, MCF, A5, A10, A20, ML), In-test (CT, CFT, MCF, A5, A10, A20, ML) and Fib-test (CT, CFT, MCF, A5, A10, A20, ML) and based on these results, establish ClotPro® reference ranges in parturients. The secondary aim is to compare the difference in coagulation parameters between pregnant women in the peripartum period and a control group of non-pregnant women (NPW) on the ClotPro® device. Furthermore, a comparison with the physiological parameters of pregnant women in the peripartum period intended for ROTEM® and TEG® devices (they are already known).

Detailed Description

This is a prospective observational cross-sectional study with a control group whose task is to describe the physiological parameters of the coagulation status of pregnant women in the peripartum period measured on the ClotPro® device. The study is monocentric and will take place at the Brno University Hospital. The study will evaluate a group of pregnant women in the peripartum period who come to give birth at the Brno University Hospital and fulfill the inclusion criteria, the target number is 120 patients. A control group of non-pregnant patients who fulfill the inclusion criteria to verify the difference between pregnant patients in the peripartum period and non-pregnant patients will also be included, the target number is 40 patients. For the participant the study is completed after obtaining the results from the ClotPro® device, there will be no reaction to result. The study is an academic research project, not sponsored by a private entity, and data will not be provided to a private entity.

The primary aim of the study is to determine the physiological parameters of the coagulation status of pregnant women in the peripartum period (PWPP) for the ClotPro® device - Ex-test (CT, CFT, MCF, A5, A10, A20, ML), In-test (CT, CFT, MCF, A5, A10, A20, ML) and Fib-test (CT, CFT, MCF, A5, A10, A20, ML) and based on these results, establish ClotPro® reference ranges in parturients. The secondary aim is to compare the difference in coagulation parameters between pregnant women in the peripartum period and a control group of non-pregnant women (NPW) on the ClotPro® device. Furthermore, a comparison with the physiological parameters of pregnant women in the peripartum period intended for ROTEM® and TEG® devices (they are already known).

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
175
Inclusion Criteria
  • Age 18 - 45 years
  • BMI 18.5 - 30.0
  • Negative pregnancy test
  • The patient is able to sign an informed consent
Exclusion Criteria
  • Antiplatelet treatment
  • Anticoagulation treatment
  • Hereditary or acquired coagulopathy
  • History of thrombosis or pulmonary embolism
  • Acute or chronic inflammation (fever, septic condition, autoimmune disease)
  • Active bleeding
  • History of hemato-oncological disease
  • Refusal of inclusion in the study by the patient

GROUP: Non-pregnant group

Inclusion Criteria:

  • A pregnant woman admitted to Department of gynecology and obstetrics for the labor at the physiological term (38th week (38+0) and more)
  • Age 18 - 45 years
  • BMI 18.5 - 30.0 (before pregnancy)
  • Blood samples are indicated for standard blood samples before labor
  • The patient is able to sign an informed consent at the time of admission

Exclusion Criteria:

  • Antiplatelet treatment
  • Anticoagulation treatment
  • Hereditary or acquired coagulopathy
  • History of thrombosis or pulmonary embolism
  • Acute or chronic inflammation (fever, septic condition, autoimmune disease)
  • Active bleeding
  • Preeclampsia
  • Eclampsia
  • Gestational diabetes
  • Abruption of the placenta
  • HELLP syndrome
  • History of hemato-oncological disease
  • Pregnancy in last 6 months
  • Refusal of inclusion in the study by the patient

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
Pregnant womenCoagulation test on device ClotProIt is a group of pregnant women after finished 37th week of gestation. We will take blood sample for a coagulation test on the device ClotPro.There is no more intervention.
Non-pregnant womenCoagulation test on device ClotProIt is a control group - non-pregnant women. We will take blood samples for blood count and coagulation status and a coagulation test on the device ClotPro.There is no more intervention.
Primary Outcome Measures
NameTimeMethod
CT for Ex-test on ClotPro® devicebefore the labor

Clotting time for Ex-test ClotPro® device

CT for In-test on ClotPro® devicebefore the labor

Clotting time for In-test ClotPro® device

CT for Fib-test on ClotPro® devicebefore the labor

Clotting time for Fib-test ClotPro® device

CFT for Ex-test on ClotPro® devicebefore the labor

Clot formation time for Ex-test ClotPro® device

CFT for In-test on ClotPro® devicebefore the labor

Clot formation time for In-test ClotPro® device

ML for Ex-test on ClotPro® devicebefore the labor

Maximum lysis for Ex-test ClotPro® device

ML for In-test on ClotPro® devicebefore the labor

Maximum lysis for In-test ClotPro® device

ML for Fib-test on ClotPro® devicebefore the labor

Maximum lysis for Fib-test ClotPro® device

Hemoglobin levelbefore the labor

Hemoglobin level

Hematocrite levelbefore the labor

Hematocrite level

Platelet levelbefore the labor

Platelet level

Leucocytes levelbefore the labor

Leucocytes level

Fibrinogen levelbefore the labor

Fibrinogen level

Prothrombin timebefore the labor

Prothrombin time

activated Partial thromboplastin timebefore the labor

activated Partial thromboplastin time

INRbefore the labor

INR

Thrombin timebefore the labor

Thrombin time

CFT for Fib-test on ClotPro® devicebefore the labor

Clot formation time for Fib-test ClotPro® device

A5 for Ex-test on ClotPro® devicebefore the labor

Maximum clot firmness in 5th minute for Ex-test ClotPro® device

A5 for In-test on ClotPro® devicebefore the labor

Maximum clot firmness in 5th minute for In-test ClotPro® device

A5 for Fib-test on ClotPro® devicebefore the labor

Maximum clot firmness in 5th minute for Fib-test ClotPro® device

A10 for Ex-test on ClotPro® devicebefore the labor

Maximum clot firmness in 10th minute for Ex-test ClotPro® device

A10 for In-test on ClotPro® devicebefore the labor

Maximum clot firmness in 10th minute for In-test ClotPro® device

A10 for Fib-test on ClotPro® devicebefore the labor

Maximum clot firmness in 10th minute for Fib-test ClotPro® device

A20 for Ex-test on ClotPro® devicebefore the labor

Maximum clot firmness in 20th minute for Ex-test ClotPro® device

A20 for In-test on ClotPro® devicebefore the labor

Maximum clot firmness in 20th minute for In-test ClotPro® device

A20 for Fib-test on ClotPro® devicebefore the labor

Maximum clot firmness in 20th minute for Fib-test ClotPro® device

MCF for Ex-test on ClotPro® devicebefore the labor

Maximum clot firmness for Ex-test ClotPro® device

MCF for In-test on ClotPro® devicebefore the labor

Maximum clot firmness for In-test ClotPro® device

MCF for Fib-test on ClotPro® devicebefore the labor

Maximum clot firmness for Fib-test ClotPro® device

Establish ClotPro® reference ranges in parturientsbefore the labor

Reference ranges will be established according to IFCC guidelines, based on physiological coagulation parameters measured in parturient women using the ClotPro® device.

Secondary Outcome Measures
NameTimeMethod
Comparison of fibrinogen level and Fib-testbefore the labor

Comparison of fibrinogen level and Fib-test

Comparison of Prothrombin time and CT in Ex-testbefore the labor

Camparison of Prothrombin time and CT in Ex-test

Comparison of activated Partial thromboplastin time and In-testbefore the labor

Comparison of activated Partial thromboplastin time and In-test

Agebefore the labor

Age

Weightbefore pregnancy

Weight

Heightbefore the labor

Height

BMIbefore pregnancy

BMI

Number of pregnanciesbefore the labor

Number of pregnancies

Multiplicity of pregnancybefore the labor

Multiplicity of pregnancy

Smokingbefore the labor

Smoking

Blood lossimmediately after the labor

Blood loss

Type of labourimmediately after the labor

spontaneous labor or cesarian section

Term of deliveryimmediately after the labor

Term of delivery

Trial Locations

Locations (1)

University Hospital Brno

🇨🇿

Brno, Czechia

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