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Effect of Fasting on Hypoglycemic Counterregulation in Type 1 Diabetes

Not Applicable
Recruiting
Conditions
Hypoglycemia
Type1diabetes
Registration Number
NCT05973799
Lead Sponsor
University of Cincinnati
Brief Summary

Iatrogenic hypoglycemia is still considered to be the number one barrier to effective glycemic control in patients with type 1 diabetes (T1D). In a previous study, it was observed in people without diabetes that fasting can be detrimental to the hormonal and hepatic responses to insulin-induced hypoglycemia. In the experiments described herein, the impact fasting has on hypoglycemic counterregulation in people with T1D will be determined.

Detailed Description

Because patients with type 1 diabetes (T1D) are required to estimate and administer their own insulin requirements, they frequently overestimate their needs. This often leads to debilitating insulin-induced hypoglycemia, which is the number one barrier to the safe, effective management of glycemia in this population. In addition to the difficulty estimating one's own insulin requirements after a meal, counterregulatory hormone responses to hypoglycemia are impaired in patients with T1D, thereby reducing hepatic glucose production (HGP) and increasing the depth and duration of the hypoglycemic episode.

The discovery of ways by which counterregulatory responses to hypoglycemia can be improved in people with T1D is a priority. In previous experiments, it was observed that fasting reduces counterregulatory hormone secretion in healthy humans during insulin-induced hypoglycemia, thereby reducing hepatic glucose production (HGP). Therefore, the studies proposed herein will determine the effect of fasting on hypoglycemic counterregulation in people with T1D. It is hypothesized that fasting will diminish the hormonal and hepatic responses to insulin-induced hypoglycemia.

Each subject will undergo two trials; one where they eat an isocaloric breakfast and lunch prior to an insulin-induced hypoglycemic challenge and a second one during which they remain fasted prior to the hypoglycemic challenge. This study design will allow assessment of the relationship between fasting and the counterregulatory responses to insulin-induced hypoglycemia in a population that is particularly vulnerable to low blood sugar.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
10
Inclusion Criteria
  • males and females of any race or ethnicity
  • non-obese (BMI < or = to 30)
  • have a diagnosis of type 1 diabetes
  • C-peptide negative
Exclusion Criteria
  • pregnant women
  • cigarette smoking
  • Taking inflammation-targeting steroids (e.g., prednisone).
  • Taking medications targeting adrenergic signaling (e.g., beta-blockers, bronchodilators).
  • Hematocrit less than 33%.
  • Presence of HIV or hepatitis (due to their deleterious effects on the liver).
  • The presence of cardiovascular or peripheral vascular disease.
  • The presence of neuropathy, retinopathy or nephropathy.
  • A detection of the presence of any other disease or condition by one of the study doctors, that would be expected to confound the responses to insulin-induced hypoglycemia or make participation in the study dangerous to the individual.

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Primary Outcome Measures
NameTimeMethod
Hepatic glucose productionDuring procedure, up to 2.5 hours

From plasma

GlucagonDuring procedure, up to 2.5 hours

From plasma

Glucose infusion rateDuring procedure, up to 2.5 hours

Amount of glucose required to maintain glycemia at \~55 mg/dL.

Secondary Outcome Measures
NameTimeMethod
Peripheral glucose uptakeDuring procedure, up to 2.5 hours

From plasma

EpinephrineDuring procedure, up to 2.5 hours

From plasma

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