Analysis of Volatile Organic Compounds in Exhaled Air and in Sweat in the Context of Suspicion of COVID-19 Infection

Not Applicable

Completed

Interventions: Device: Volatile Organic Compounds analysis
Other: Canine odor detection of Volatile Organic Compounds

Brief Summary: In the context of the actual pandemia of the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which requires a better diagnostic strategy for the management of patients.

The study of volatile organic compounds (VOC) detected in exhaled air or in sweat, is an innovative research area for respiratory diseases. The analysis of VOC can be done either by the technique of the mass spectrometry which allows the identification of each VOC in the exhaled air or by the technique of electronic nose, simpler and faster, which provides an idea of the general profile of the VOC without identifying them. The VOC have shown their interest in some situations, such as diagnostic or prognostic tool in patients followed for thoracic tumorous pathology or bronchial or pulmonary vascular diseases.

Moreover, it has recently been shown that properly trained dogs would be able to detect an olfactory signature of SARS-CoV-2 infection with a specificity greater than 90%; this olfactory signature corresponds to VOCs detectable by the flair of dogs (Nosaïs-Covid19 study).

Validation of the diagnostic value of VOC analyzes by non-invasive and rapid methods (electronic nose analysis or mass spectrometry; detection by the scent of dogs) for the rapid detection and early diagnosis of a SARS-CoV-2 infection warrants the performance of this clinical study.

Recruitment & Eligibility

Inclusion Criteria

Exclusion Criteria

Arm && Interventions

Group	Intervention	Description
Symptomatic patients with positive PCR	Volatile Organic Compounds analysis	Patients with symptoms of COVID-19 and whose PCR result is positive
Asymptomatic patients or Healthy volunteers	Canine odor detection of Volatile Organic Compounds	Patient with no symptom of COVID-19 infection but for whom a PCR test needs to be done because he has been in contact with a COVID-19 positive person
Symptomatic patients with positive PCR	Canine odor detection of Volatile Organic Compounds	Patients with symptoms of COVID-19 and whose PCR result is positive
Symptomatic patients with negative PCR but with seroconversion within 4 to 8 weeks	Volatile Organic Compounds analysis	Patients with symptoms of COVID-19 and whose PCR result is negative at inclusion but presents a seroconversion within 4 to 8 weeks post inclusion
Symptomatic patients with negative PCR but with seroconversion within 4 to 8 weeks	Canine odor detection of Volatile Organic Compounds	Patients with symptoms of COVID-19 and whose PCR result is negative at inclusion but presents a seroconversion within 4 to 8 weeks post inclusion
Asymptomatic patients or Healthy volunteers	Volatile Organic Compounds analysis	Patient with no symptom of COVID-19 infection but for whom a PCR test needs to be done because he has been in contact with a COVID-19 positive person

Primary Outcome Measures

Name	Time	Method
Validate the interest of VOCs analyzed by electronic noses and / or mass spectrometry for the diagnosis of a COVID-19 infection	1 day	Comparison of variation of Volatile Organic Compound (VOC) profiles in exhaled air of patients between patients with symptoms suggestive of COVID and infection confirmed by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and those whose infection is ruled out based on the negativity of RT-PCR and clinical data

Secondary Outcome Measures

Name	Time	Method
Assessment of the specificity and sensitivity of canine olfactory detection of COVs associated with infection by SARS-CoV-2 (COVID-19)	1 day	Proportion of success in detecting samples from patients with symptoms suggestive of COVID and infection confirmed by PCR.
Identify the nature of VOCs associated with infection by SARS-CoV-2 by mass spectrometry in exhaled air and in sweat	1 day	The equivalences and differences in nature of VOCs associated with infection with SARS-CoV-2 between exhaled air and sweat.