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The Transition From Blastomere to Trophectoderm Biopsy: Comparing Two PGS Strategies

Completed
Conditions
Preimplantation Genetic Screening
Infertility
Registration Number
NCT03062969
Lead Sponsor
Institut Universitari Dexeus
Brief Summary

Shortly after the implementation of Comprehensive Chromosome Screening (CCS) techniques for Preimplantation Genetic Screening (PGS) came the transition in biopsy timing. Trophectoderm biopsy is meant to overcome the limitations of cleavage stage biopsy and single cell analysis.

Detailed Description

The investigators have recently introduced this new approach and a frozen-thawed policy of euploid blastocysts is routinely performed.

This is a single centre retrospective study in patients undergoing a PGS cycle. The aim of the study is to assess the results obtained in a PGS programme after the implementation of a new biopsy timing and transfer policy. Results obtained in PGS cycles with blastocyst biopsy and frozen-thawed embryo transfer were analysed in comparison to cycles with cleavage stage biopsy and fresh blastocyst transfer.

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
383
Inclusion Criteria
  • Own gametes
  • PGS for: advanced maternal age, repeated implantation failure, recurrent miscarriages, severe male factor, and previous affected pregnancies
Exclusion Criteria
  • PGS cycles with gamete donation
  • Altered karyotypes
  • Combined Preimplantation genetic diagnosis (PGD)+PGS cycles

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Ongoing clinical pregnancy rate20 weeks after embryo transfer

Clinical pregnancy confirmed by ultrasound

Secondary Outcome Measures
NameTimeMethod
Number of available euploid blastocysts per cycleUp to 15 days after embryo biopsy

Transferable or vitrified euploid blastocysts

Miscarriage RateUntil 20 weeks after embryo transfer
Implantation Rate6 weeks after embryo transfer

Embryos implanted confirmed by ultrasound

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