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Impact of Milk Fat Globule Membrane Supplementation on Heart and Brain Health

Not Applicable
Completed
Conditions
Overweight and Obesity
Cognition
Cardiovascular Diseases
Registration Number
NCT05939544
Lead Sponsor
Loughborough University
Brief Summary

In a randomised, controlled cross-over manner, this trial aims to determine how short-term daily supplementation with a milk fat globule membrane-enriched ingredient impacts on cardiometabolic health and cognitive outcomes.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
18
Inclusion Criteria
  • Aged 40 - 70 years
  • BMI: 25-45 kg/m2
  • Recreationally active (> 3 x 30 min moderate exercise per week)
  • Understands and is willing and able to comply with all study procedures, including changes to diet
  • Fluent in written and spoken English
  • Access to, and able to use, the internet/computer/tablet device
Exclusion Criteria
  • Smoking (including vaping)
  • Unstable weight history (≥3 kg loss or gain in the previous 3 months) or planning or currently on a weight reduction scheme
  • Currently taking part or have participated in another research study in the last two months (e.g., dietary intervention)
  • Diagnosed with cardiovascular disease or suffered myocardial infarction/stroke in the past twelve months
  • Existing or significant past medical history of any medical condition likely to affect the study outcomes.
  • Prescribed medications or supplements likely to interfere with study outcomes or prescribed antibiotics within the last three months
  • Use of antidepressant/anti-anxiety medication if it has changed in the last three months or expected to change within the 3-month study period.
  • Known allergy or intolerance to study food (lactose intolerance, dairy)
  • Being vegan or any other unusual medical history or diet and lifestyle habits or practices that would preclude volunteers from participating in a dietary intervention or metabolic study
  • Excessive alcohol consumption: >21 unit/wk
  • Pregnancy, seeking to become pregnant or active lactation

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Primary Outcome Measures
NameTimeMethod
Change-from-baseline in circulating LDL-cholesterol concentrations28 days

Assessed following the collection of fasted blood samples before and after each 28-day study period

Secondary Outcome Measures
NameTimeMethod
Change-from-baseline in glucose concentrations (determined by spectrophotometric assay)28 days

Assessed following the collection of fasted blood samples before and after each 28-day study period

Change-from-baseline in clinic systolic and diastolic blood pressure (assessed by automated upper arm sphygmomanometer)28 days

Assessed in the fasted state before and after each 28-day study period

Change-from-baseline in cognitive test performance using a neuropsychological seven-test battery which assesses global and domain specific function, as determined by NeurOn software28 days

Assessed before and after each 28-day study period

Change-from-baseline in body mass (kg) using standard equipment.28 days

Assessed in the fasted state before and after each 28-day study period

Change-from-baseline in waist circumference (cm) using standard equipment.28 days

Assessed in the fasted state before and after each 28-day study period

Change-from-baseline in brain-derived neurotrophic factor concentrations (determined by ELISA)28 days

Assessed in the fasted state before and after each 28-day study period

Change-from-baseline in interleukin-6 concentrations (determined by ELISA)28 days

Assessed following the collection of fasted blood samples before and after each 28-day study period

Change-from-baseline in insulin concentrations (determined by ELISA)28 days

Assessed following the collection of fasted blood samples before and after each 28-day study period

Change-from-baseline in circulating lipid and apolipoprotein concentrations (measured using a spectrophotometric assay or high-throughput 1H-NMR metabolomics platform)28 days

Assessed following the collection of fasted blood samples before and after each 28-day study period

Change-from-baseline in particle size of lipoprotein subclasses, and concentrations of particles within each subclass by high-throughput 1H-NMR metabolomics platform28 days

Assessed following the collection of fasted blood samples before and after each 28-day study period

Change-from-baseline in selected gut-related metabolites (for example, trimethylamine N-oxide concentrations determined by mass spectrometry)28 days

Assessed following the collection of fasted blood samples before and after each 28-day study period

Change-from-baseline in HOMA-IR (calculated as [fasting glucose (mmol/L) × fasting insulin (mIU/L)]/22.5])28 days

Assessed following assessment of fasting circulating glucose and insulin concentrations before and after each 28-day study period

Change-from-baseline in lipopolysaccharide-binding protein concentrations (determined by ELISA)28 days

Assessed following the collection of fasted blood samples before and after each 28-day study period

Change-from-baseline in arterial stiffness, as assessed by radial pulse wave analysis (using applanation tonometry)28 days

Assessed in the fasted state before and after each 28-day study period

Change-from-baseline in forearm blood flow, as assessed by venous occlusion plethysmography (using applanation tonometry)28 days

Assessed in the fasted state before and after each 28-day study period

Change-from-baseline in body fat (%) using standard equipment.28 days

Assessed in the fasted state before and after each 28-day study period

Trial Locations

Locations (1)

Loughborough University

🇬🇧

Loughborough, United Kingdom

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