Molecular Diagnostic Accuracy and Novel Detection of Dengue Virus Using an NS2 Gene–Based Reverse Transcription Loop-Mediated Isothermal Amplification Assay

This study aims to evaluate the diagnostic accuracy of a novel NS2 gene based reverse transcription loop mediated isothermal amplification assay for detection of dengue virus. Clinically suspected dengue patients aged 0 to 65 years will be included and clinical samples will be tested using the DEN LAMP assay and compared with conventional RT PCR as the reference standard. The study will assess sensitivity specificity predictive values time to detection analytical performance operational feasibility cost per test and agreement between the two methods to determine the usefulness of DEN LAMP as a rapid and feasible diagnostic tool for dengue infection.