PPAR-γ, RXR, VDR, and NF-κB Expressions in Gingival Tissue Samples of Healthy
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 45
- 试验地点
- 1
- 主要终点
- histological tissue evaluation
研究概览
简要总结
Objective: Diabetes and periodontitis are two chronic inflammatory diseases sharing specific etiopathogenetic mechanisms, and both cause severe inflammation and destruction. The aim of the present study was to determine the receptor expressions of Peroxisome proliferative-activated receptor (PPAR)-γ, Retinoid X receptor (RXR)-α, Vitamin D receptor (VDR), and nuclear factor kappa B (NF-κB) expressions in healthy gingiva and diseased gingival samples with diabetes.
Methods: 45 participants as 1; healthy controls (C), 2; periodontitis group (P), and 3; diabetes and periodontitis group (DP) were enrolled. Plaque index (PI), gingival index (GI) and clinical attachment levels (CAL) were recorded in all participants. Two gingival biopsies from each participant were obtained, and one underwent histological tissue processing while the other underwent RT-PCR analysis of nuclear receptors. Inflammatory and fibroblast cell counts, PPAR-γ, RXR-α, and VDR were evaluated.
Results: Fibroblast cells were lowest in the DP group and highest in the healthy group. PPAR-γ, VDR, RXR, and NF-κB expressions were higher in the healthy controls in the RT-PCR analysis and similar in the other groups. Immunohistochemistry analysis also showed similar results.
Conclusion: Results concluded that healthy gingival samples had higher PPAR-γ, RXR, VDR, and NF-κB expressions, and the immunohistochemistry results also supported this finding. The healthy gingiva contained higher fibroblast cells and lower inflammatory cells.
详细描述
The present study protocol was approved by the ethical committee of the Medical Ethics Committee of Tokat Gaziosmanpasa University. The study was designed as a cross-sectional clinical study and conducted at Tokat Gaziosmanpaşa University Faculty of Dentistry Department of Periodontology. Informed consent was obtained from all participants, and detailed oral and radiological examination was performed by an experienced clinician (H.B.Y.).
Forty-two participants, 14 participants in each group, were enrolled in the study. The study groups were as follows.
Healthy controls (C, mean age 41.05±1.80, seven men, seven women) Periodontitis patients with stage 3 grade B, (P, mean age 42.35±1.92, seven men, seven women) Patients with diabetes and periodontitis with stage 3 grade C, (DP, mean age 41.09±1.28, seven men, seven women)
All patients and healthy individuals went through a detailed oral examination, and periodontal health and periodontitis diagnosis were based on the criteria defined by the recent classification of periodontal health and diseases in the 2017 International World Workshop for a Classification of Periodontal Diseases and Conditions [21].
All participants were never smokers. Exclusion criteria were conditions which might affect the inflammatory state, the existence of less than ten or over missing teeth, tobacco or any drug use, presence of pregnancy or lactation, and existence of antibiotic or periodontal therapy within previous six months. Diabetes patients in the present study had HbA1c values greater than 7.0 even though they were under diabetic treatment. And the diabetic patients who had HbA1c values below 7.0 were not included.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Only
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Periodontally healthy
排除标准
- •Inflammatory state
- •The existence of less than ten or over missing teeth
结局指标
主要结局
histological tissue evaluation
时间窗: 1 month.
Inflammatory and fibroblast cell counts, PPAR-γ, RXR-α, and VDR were evaluated.
次要结局
未报告次要终点
研究者
mehmet murat taskan
Assistant Professor
Tokat Gaziosmanpasa University