PPAR, RXR, and VDR Expressions in Peri-implantitis

Completed

• Conditions: Periodontitis; Periimplantitis
• Interventions: Other: Gingival biopsies

• Registration Number: NCT04020146
• Lead Sponsor: Tokat Gaziosmanpasa University

Brief Summary

ABSTRACT Objective: Periodontitis and peri-implantitis are the irreversible destructive diseases of the periodontal and peri-implant tissues. The present study aimed to determine the receptor expressions of Peroxisome proliferative-activated receptor (PPAR)-γ, Retinoid X receptor (RXR)-α, and Vitamin D receptor (VDR) in the diseased tissues around teeth and dental implants.

Methods: The study consisted of three groups as group 1; healthy controls (C, n=15), group 2; periodontitis patients with stage 3 grade B (P, n=15), and group 3; peri-implantitis patients (PI, n=15). Periodontal clinical parameters as the plaque index (PI), gingival index (GI) and clinical attachment levels (CAL) were recorded. Gingival biopsies were obtained from all participants and biopsy samples underwent histological tissue processing. Hematoxylin-eosin (H\&E) and immunohistochemistry staining were performed. Total inflammatory cell counts and fibroblast cell density were evaluated on H\&E-stained slides while PPAR-γ, RXR-α, and VDR were evaluated via immunohistochemistry.

Detailed Description

Not available

Study Timeline

• Study Start: 2018-01-10
• Primary Completion: 2019-01-10
• Study Completion: 2019-03-10
• Status Verified: 2019-07
• Study First Submitted: 2019-07-08
• Study First Submitted QC: 2019-07-12
• Last Update Submitted: 2019-07-12
• Study First Posted: 2019-07-15
• Last Update Posted: 2019-07-15

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 45

Inclusion Criteria

• healthy individuals
• periodontitis patients
• periimplantitis patients

Exclusion Criteria

• smokers,
• systemically compromised patients

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
group 1;	Gingival biopsies	healthy controls (C, n=15),
group 3	Gingival biopsies	peri-implantitis patients (PI, n=15).
group 2	Gingival biopsies	periodontitis patients with stage 3 grade B; (P, n=15)

Primary Outcome Measures

Name	Time	Method
Fibroblast counts	6 months	Fibroblasts counts were evaluated from H\&E stained slides under 1000x magnification via a light microscope (Nikon Eclipse, E 600, Tokyo, Japan).
Inflammatory cells counts	6 months	Gingival biopsies were obtained from all participants and biopsy samples underwent histological tissue processing. Hematoxylin-eosin (H\&E) staining were performed. Total inflammatory cell counts density were evaluated on H\&E-stained slides.A cell counting frame of 10.000 µm2 area was selected under 1000x magnification. The total inflammatory cells (neutrophil, lymphocyte, eosinophil, and macrophage cells) within the frame were counted as inflammatory cell counting.
Peroxisome proliferative-activated receptor (PPAR)-γ	During histological processing	Gingival biopsies were obtained from all participants and biopsy samples underwent histological tissue processin and immunohistochemistry staining were performed, PPAR-γ, was evaluated via immunohistochemistry. Samples were examined under 400x magnification using light microscopy (Nikon Eclipse, E 600, Tokyo, Japan).
Vitamin D receptor (VDR)	6 months	. Gingival biopsies were obtained from all participants and biopsy samples underwent histological tissue processing and immunohistochemistry staining were performed, VDR was evaluated via immunohistochemistry. Samples were examined under 400x magnification using light microscopy (Nikon Eclipse, E 600, Tokyo, Japan).
Retinoid X receptor (RXR)-α	6 months	Gingival biopsies were obtained from all participants and biopsy samples underwent histological tissue processing and immunohistochemistry staining were performed, RXR-α was evaluated via immunohistochemistry. Samples were examined under 400x magnification using light microscopy (Nikon Eclipse, E 600, Tokyo, Japan).

Trial Locations

Locations (1)

Gaziosmanpasa University; 🇹🇷 Tokat, Turkey