Running Title: PPAR-γ, RXR-α, and VDR Expressions in Periodontitis

• Conditions: Periodontitis
• Interventions: Diagnostic Test: Gingiva Biopsy Sampling

• Registration Number: NCT04018144
• Lead Sponsor: Tokat Gaziosmanpasa University

Brief Summary

Objective: The present study aimed to determine the expressions of nuclear receptors PPAR (Peroxisome proliferative activator receptor)-γ, RXR (Retinoid X receptor)-α, and vitamin D receptor (VDR) in healthy volunteers and periodontitis patients with different grade involvement.

Methods: Group-1; healthy individuals, Group-2; periodontitis patients-stage 3 grade B, (H-GradeB), Group-3; periodontitis patients-stage 3 grade C, (D-GradeC). Clinical parameters as plaque index (PI), gingival index (GI) and clinical attachment levels (CAL) were measured. Fibroblast and inflammatory cells, PPAR-γ, RXR-α, and VDR levels were determined in histological slides.

Detailed Description

Not available

Study Timeline

• Status Verified: 2019-07
• Study First Submitted: 2019-07-10
• Study First Submitted QC: 2019-07-10
• Last Update Submitted: 2019-07-10
• Study First Posted: 2019-07-12
• Last Update Posted: 2019-07-12
• Study Start: 2019-08-01
• Primary Completion: 2019-12-01
• Study Completion: 2020-03-01

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 45

Inclusion Criteria

• systemic health
• no drug use
• no previous periodontal treatment within six months
• no tobacco use

Exclusion Criteria

• pregnant or lactating women
• patients used antibiotics in the last 6 months

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Group-2	Gingiva Biopsy Sampling	Periodontitis patients-stage 3-grade B
Group-3	Gingiva Biopsy Sampling	Periodontitis patients-stage 3-grade C
Group-1	Gingiva Biopsy Sampling	Healthy individuals

Primary Outcome Measures

Name	Time	Method
Fibroblasts counts	During histological tissue processing	For cell counting, connective tissue neighboring gingival epithelium was marked, and a cell counting frame of 10000 µm2 was marked. The fibroblast counts within the frame were counted. The measurements were performed from three different points, and the mean of these three measurements was recorded.
Vitamin D receptor (VDR)	During histological tissue processing	Vitamin D receptor (VDR) levels were determined via immunohistochemistry.
Inflammatory cell counts	During histological tissue processing	Connective tissues were evaluated, and inflammatory cell infiltration were counted in the H\&E stained slides using a light microscope (Nikon Eclipse, E 600, Tokyo, Japan). For cell counting, connective tissue neighboring gingival epithelium was marked, and a cell counting frame of 10000 µm2 was marked. Inflammatory cells (neutrophil, lymphocyte, eosinophil, and macrophage cells) within the frame were counted. The measurements were performed from three different points, and the mean of these three measurements was recorded.
PPAR (Peroxisome proliferative activator receptor)-γ	During histological tissue processing	PPAR (Peroxisome proliferative activator receptor)-γ levels were determined via immunohistochemistry.
RXR (Retinoid X receptor)-α	During histological tissue processing	RXR (Retinoid X receptor)-α levels were determined via immunohistochemistry.

Trial Locations

Locations (1)

Gaziosmanpasa University; 🇹🇷 Tokat, Turkey