Epigenetic Effects of a Single Bout of Exercise on Cardiovascular Risk Factors and the Metabolome in Lean and Overweight or Obese Subjects
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 30
- 试验地点
- 2
- 主要终点
- Change from baseline in skeletal transcriptome
研究概览
简要总结
The purpose of this study is to determine the ability of acute exercise to regulate fat metabolism in muscle of overweight and obese people compared to lean people.
详细描述
After being informed about the study details and possible risks and benefits, 15 overweight/obese and 15 lean participants gave written informed consent and were then screened and enrolled in the study. All participants then gave a muscle sample and ate a calorically controlled breakfast. Participants completed ~1 hour of cycling exercise on a stationary bike and gave a second muscle sample 4 hours after eating breakfast.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- Single (Outcomes Assessor)
盲法说明
Investigators conducting molecular analysis were blinded to study samples/groups.
入排标准
- 年龄范围
- 18 Years 至 30 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •healthy males that completed a physical examination with a State Licensed M.D. or D.O. within the past 2 years and completed a physical activity readiness questionnaire (PARQ), the International Physical Activity Questionnaires (IPAQ) and and a medical history questionnaire prior to the study
排除标准
- •any participants that had not had a physical examination by a State Licensed M.D. or D.O. within the past 2 years
- •persons not considered "healthy" (with any previous medical diagnoses) or not medically cleared by a M.D. or D.O. to perform physical activity/exercise
- •persons who are regularly physically active
结局指标
主要结局
Change from baseline in skeletal transcriptome
时间窗: Baseline and immediately after acute cycling exercise
RNA-seq is a method used to quantify changes in all known messenger RNA levels in the genome, giving an assessment of whole genome gene transcription changes.
Change from baseline in skeletal muscle acylcarnitines
时间窗: Baseline and immediately after acute cycling exercise
Metabolomics of skeletal muscle acylcarnitines is used as a measure of mitochondrial function by assessing long, medium and short chain acylcarnitine levels.
Change from baseline in skeletal muscle whole genome nucleosome maps
时间窗: Baseline and immediately after acute cycling exercise
MNase-seq is a method used to determine the occupancy/location of nucleosomes throughout the entire genome, supporting evidence of alterations in epigenetic modifications occurring at specific genomic loci that affect nucleosome occupancy/location and possibly gene transcription or silencing.
次要结局
- Triglycerides(Baseline and immediately after acute cycling exercise)
- Glucose(Baseline and immediately after acute cycling exercise)
- Free fatty acids(Baseline and immediately after acute cycling exercise)
- Insulin(Baseline and immediately after acute cycling exercise)
- Cholesterol(Baseline and immediately after acute cycling exercise)
研究者
Jessica Ellis
Assistant Professor
East Carolina University