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Identify Biomarkers of Tobacco Exposure and Nicotine Uptake From JUUL 5% in Adult Smokers

Not Applicable
Completed
Conditions
Nicotine Dependence, Other Tobacco Product
Smoking, Tobacco
Tobacco Use
Interventions
Other: Treatment with JUUL 5%, Virginia Tobacco
Other: Treatment with JUUL 5%, Cool Mint, ENDS
Other: Smoking Cessation
Other: Combustible cigarette
Other: JUUL 5%, Creme Bruele, ENDS
Other: Treatment with JUUL 5%, Mango, ENDS
Registration Number
NCT03463837
Lead Sponsor
Juul Labs, Inc.
Brief Summary

The JUUL 5% Electronic Nicotine Delivery System (ENDS) is being studied as an alternative to combustible cigarette use. This study aims to find out how much nicotine is in the blood and urine of healthy adult subjects after using three JUUL 5% ENDS compared to smoking usual brands of combustible cigarettes and stopping smoking.

Detailed Description

This study will serve as a clinical evaluation of the exclusive use of selected JUUL ENDS, with the purpose of gaining an understanding of toxicant exposure.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
90
Inclusion Criteria
  1. Healthy, adult, male or female smoker, 21 to 65 years of age.
  2. Has been a smoker for at least 12 months prior to Screening.
  3. Currently smokes an average of 10 cigarettes each day.
  4. Has a positive urine cotinine (≥ 500 ng/mL).
  5. Has an exhaled carbon monoxide (CO) > 12 ppm.
  6. A female subject of childbearing potential must have been using contraception and agree to continue using it through completion of the study:
  7. Provides voluntary consent to participate in this study documented on the signed informed consent form (ICF).

Key Exclusion Criteria

  1. Has a history or presence of clinically significant conditions.
  2. Has a body mass index (BMI) > 40 kg/m2 or < 18 kg/m2 at Screen
  3. Has a history of drug or alcohol abuse
  4. Has an estimated creatinine clearance < 80 mL/minute
  5. If female, the subject is pregnant, lactating, or intends to become pregnant during the time period from Screening through the end of study.
  6. Has used nicotine-containing products other than manufactured cigarettes within 14days of screening.
  7. Is planning to quit smoking during the study, planning to quit within 3 months following Day 1
Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Treatment with JUUL 5%, Virginia TobaccoTreatment with JUUL 5%, Virginia TobaccoJUUL 5%,Virginia Tobacco \[5 days\] in confinement.
Treatment with JUUL 5%, Cool Mint, ENDSTreatment with JUUL 5%, Cool Mint, ENDSJUUL 5%, Cool Mint \[5 days\] in confinement.
Smoking cessation (no smoking)Smoking CessationSmoking cessation (no smoking).
Combustible cigaretteCombustible cigaretteExclusive use of combustible cigarette \[5 days\] in confinement.
JUUL 5%, Creme Bruele, ENDSJUUL 5%, Creme Bruele, ENDSJUUL 5%, Creme Bruele \[5 days\] in confinement.
Treatment with JUUL 5%, Mango, ENDSTreatment with JUUL 5%, Mango, ENDSJUUL 5%, Mango \[5 days\] in confinement.
Primary Outcome Measures
NameTimeMethod
Change in primary biomarkers day 5 values for each JUUL 5% product will be compared to baseline:[Time Frame: 5 days]

Change in ; Urine N nitrosonornicotine (NNN), 4 (methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL), 3 hydroxypropylmercapturic acid (3-HPMA), monohydroxybutenylmercapturic acid (MHBMA), and S-phenyl mercapturic acid (S-PMA) "Blood carboxyhemoglobin (COHb)

Secondary Outcome Measures
NameTimeMethod
Change in concentration of Nicotine in blood[Time Frame: 5 days]

Change in Biomarker

Change of area under the curve (AUC) Plasma PK of Nicotine[Time Frame: Day 7,

Nicotine absorption

Change in concentration of 3-hydroxy-1-methylpropylmercapturic acid (HMPMA) in urine[Time Frame: 5 days]

Change in Biomarker

Change in concentration of 2-cyanoethyl-mercapturic acid (CEMA) in urine[Time Frame: 5 days]

Change in Biomarker

Change in concentration of trans-3'-hydroxycotinine in urine[Time Frame: 5 days]

Change in Biomarker

Change in concentration of Glucuronides in urine[Time Frame: 5 days]

Change in Biomarker

Change in maximum concentration (Cmax) Plasma pharmacokinetic (PK) of Nicotine[Time Frame: Day 7,

Nicotine absorption

Change in concentration of Cotinine in urine[Time Frame: 5 days]

Change in Biomarker

Change in concentration of Nicotine in urine[Time Frame: 5 days]

Change in Biomarker

Change in concentration of Cotinine in blood[Time Frame: 5 days]

Change in Biomarker

Change in concentration of 1-hydroxypyrene (1-OHP) in urine[Time Frame: 5 days]

Change in Biomarker

Change in time to maximum concentration (Tmax) Plasma PK of Nicotine[Time Frame: Day 7,

Nicotine absorption

Measure of future intent to use JUUL 5% Device device relative to usual combustible cigarette and smoking cessation.[Time Frame: 6-7 days, intervention dependent]

Change in Intent

Change in concentration of trans-3'-hydroxycotinine in blood[Time Frame: 5 days

Change in Biomarker

Incidence of JUUL 5% Device, including malfunction and/or misuse[Time Frame: 7 days]

Device performance

Product use over 5-day period and during 5-minute fixed and ad libitum use episodes[Time Frame: 7 days]

Change in product use

Measure of subjective Smoking Urge Assessment[Time Frame: 6-7 days, intervention dependent]

Change in Assessment

Measure of subjective product evaluation using the "modified Product Evaluation Scale (mPES)"[Time Frame: 6-7 days, intervention dependent]

Change in Evaluation; Scale: 1 = not at all, 2 = very little, 3 = a little, 4 = moderately, 5 = a lot, 6 = quite a lot, 7 = extremely

Four multi-item subscales will be derived from "Satisfaction" (items 1, 2, 3, and 12); "Psychological Reward" (items 4 through 8); "Aversion" (items 9, 10, 16, and 18); and "Relief" (item 11, 13, 14, 15, and reversed for item 19) and single items 17 and 20 will be summarized.

Number of participants with Adverse Events associated with use of JUUL 5% Device to combustible cigarette and smoking cessation[Time Frame: 13-14 days, intervention dependent]

Safety

Trial Locations

Locations (1)

Celerion

🇺🇸

Lincoln, Nebraska, United States

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