Mechanisms of Anabolic Resistance in Older Humans
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 发起方
- Mayo Clinic
- 入组人数
- 120
- 试验地点
- 1
- 主要终点
- Anabolic response
研究概览
简要总结
The purpose of this research is to understand how chronic inflammation affects muscle function and responses to exercise in older adults.
详细描述
Inflamed adipose tissue may contribute to blunted exercise response in skeletal muscle of older adults. The objective of this project is to evaluate a hypothesis that inflamed adipose secretes factors that activate inflammatory cascades in skeletal muscle, which may interfere with exercise-responsive molecular pathways and contribute to dysfunctional muscle phenotypes with aging. This project determine how adipose tissue influences skeletal muscle function and anabolic response to exercise in older adults. Young and older adults will complete studies to assess molecular response to acute exercise from protein synthesis rates, mRNA of exercise-responsive genes, and activation of signaling proteins in skeletal muscle. Adipose tissue will be assessed using a combination of non-invasive imaging and biopsy-based molecular phenotyping. The project will determine if acute exercise response is attenuated in older adults with inflamed adipose tissue phenotype.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 21 Years 至 85 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Exclusion Criteria:
- •Diabetes or fasting plasma glucose 126 mg/dL
- •Body mass index (BMI) 30 kg/m2
- •Anemia (female subjects hemoglobin of <11 g/dl and male subjects hemoglobin <12 g/dl)
- •Active coronary artery disease or history of unstable macrovascular disease (unstable angina, myocardial infarction, stroke, and revascularization of coronary, peripheral or carotid artery within 3 months of recruitment)
- •Renal failure (serum creatinine > 1.5mg/dl)
- •Chronic active liver disease (AST>144IU/L or ALT>165IU/L)
- •Oral warfarin group medications or history of blood clotting disorders.
- •Pregnancy or breastfeeding
- •Alcohol consumption greater than 2 glasses/day or other substance abuse
- •Untreated or uncontrolled hypothyroidism
- •Debilitating chronic disease (at the discretion of the investigators)
排除标准
- •Diabetes or fasting plasma glucose 126 mg/dL
- •Body mass index (BMI) 30 kg/m2
- •Anemia (female subjects hemoglobin of <11 g/dl and male subjects hemoglobin <12 g/dl)
- •Active coronary artery disease or history of unstable macrovascular disease (unstable angina, myocardial infarction, stroke, and revascularization of coronary, peripheral or carotid artery within 3 months of recruitment)
- •Renal failure (serum creatinine > 1.5mg/dl)
- •Chronic active liver disease (AST>144IU/L or ALT>165IU/L)
- •Oral warfarin group medications or history of blood clotting disorders.
- •Pregnancy or breastfeeding
- •Alcohol consumption greater than 2 glasses/day or other substance abuse
- •Untreated or uncontrolled hypothyroidism
- •Debilitating chronic disease (at the discretion of the investigators)
结局指标
主要结局
Anabolic response
时间窗: 2 months
Skeletal muscle protein synthesis rates will be measured from isotopic enrichment of muscle proteins
次要结局
未报告次要终点