Muscle Insulin Resistance In Aging
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Insulin Resistance
- Sponsor
- AdventHealth Translational Research Institute
- Enrollment
- 200
- Locations
- 1
- Primary Endpoint
- Measure of intramyocellular lipids
- Status
- Active, not recruiting
- Last Updated
- 9 months ago
Overview
Brief Summary
The purpose of this study is to provide information regarding potential factors underlying metabolic dysfunction, insulin resistance, and loss of muscle mass in aging muscle.
Detailed Description
Study Objectives: 1. To determine the effects of diet-induced weight loss with and without the addition of exercise on mitochondrial biogenesis and energetic capacity, cellular redox state and insulin resistance. 2. To determine the effects of diet-induced weight loss with and without the addition of exercise on intramyocellular lipid profiles. 3. To determine the effects of diet-induced weight loss with and without exercise on skeletal muscle proteins mediating a program of autophagy and either loss or maintenance of muscle mass.
Investigators
Eligibility Criteria
Inclusion Criteria
- •65-80 years of age
- •Stable weight (No Gain/Loss of \>10 lbs in 6 months)
- •Sedentary (≤ 1 continuous exercise/week)
- •Non-smoker
- •BMI ≥ 30 kg/m2
- •Resting Blood Pressure ≤ 150 millimeters of mercury systolic and ≤ 95 millimeters of mercury diastolic
- •Note from Primary care physician/Cardiologist for exercise clearance if positive stress test symptoms were observed from exercise test
- •Must be willing to washout for 14 days from all diabetes medication and independent in self blood glucose monitoring during the washout periods (those with diabetes only)
Exclusion Criteria
- •Clinically significant cardiovascular disease including history of myocardial infarction, within the past year
- •Peripheral Vascular Disease
- •Hepatic, renal, muscular/neuromuscular, or active hematologic/oncologic disease
- •Clinically diminished pulse
- •Presence of bruits in lower extremities
- •Previous history of pulmonary emboli
- •Peripheral Neuropathy
Outcomes
Primary Outcomes
Measure of intramyocellular lipids
Time Frame: Visit 3 ~22 hours
A percutaneous muscle biopsy of the vastus lateralis will be obtained, and the intramyocellular lipids will be measured by high-performance liquid chromatography-tandem mass spectrometry.
Secondary Outcomes
- Measure of insulin sensitivity(Visit 3 ~22 hours)