Association of Dysbiosis and Immune Response in Bronchiolitis in Under 12 Months -Old Infants
Trial Snapshot
- Phase
- Not Applicable
- Status
- Recruiting
- Enrollment
- 120
- Locations
- 3
- Primary Endpoint
- Dysbiosis
Study Overview
Brief Summary
Acute bronchiolitis is a common disease in children under the age of two, caused mainly by the respiratory syncytial virus (RSV). Furthermore, given the same medical history, it is still very difficult to predict the course and severity of the infection at the onset of symptoms, Some studies have highlighted the importance of the microbiota (intestinal, oral or nasopharyngeal) and of the immune response to RSV in children, We will include 80 children under 2 years old with hospitalized bronchiolitis and non-hospitalized bronchiolitis. Oral, nasal and stool samples will be taken to study the various microbiota in search of dysbiosis. A capillary blood sample will be taken for immune studies.
Study Design
- Study Type
- Observational
- Observational Model
- Cohort
- Time Perspective
- Prospective
Eligibility Criteria
- Ages
- 1 Day to 12 Months (Child)
- Sex
- All
- Accepts Healthy Volunteers
- No
Inclusion Criteria
- •Infants <12 months
- •With bronchiolitis during RSV epidemic season
- •No chronic illness
- •No bronchiolitis medical history
- •Signed consent from parents or legal guardians
Exclusion Criteria
- •Chronic respiratory illness
- •Medical history of bronchiolitis or newborn asthma
- •Treatment with immunosuppressants
- •Patient with no social security affiliation
Outcomes
Primary Outcomes
Dysbiosis
Time Frame: Inclusion
comparison of the quantitative and qualitative composition of bacteria (alpha diversity, beta diversity, Shannon and Simpson index) in the digestive and nasopharyngeal microbiota
Secondary Outcomes
- Measurement of innate and adaptive responses by quantification of cytokines and chemokines in plasma(inclusion)
- Identifying the mRNA profile in blood samples and nasal swabs(inclusion)
- Sequencing viral strains for mutation(Inclusion)
- Comparison of respiratory syncytial virus (RSV) antibodies levels on newborn screening specimen and on capillary swab during infection(Birth, Inclusion)
- Study the load of Streptococcus pneumoniae (Sp) in RSV infections.(Inclusion)