Characterization of Obesity-related Cardiomyopathy Through Exploration of Human Atrial Trabeculae Contraction

Completed

• Conditions: Heart Failure; Obesity
• Interventions: Other: functional tissu atrial analysis

• Registration Number: NCT02384252
• Lead Sponsor: University Hospital, Lille

Brief Summary

The investigators aim to explore obese cardiomyopathy by studying contractile twitch force, sarcomere sensitivity to calcium and mitochondrial function in atrial myocardial samples of patients grouped according to their body mass index, i.e. normal weight, overweight and obese.

Detailed Description

Obesity is associated with the development of heart failure, regardless of coronary artery disease or other comorbidities.

Right atrial samples obtained from patients undergoing cardiac surgery and presenting no sign of clinical cardiomyopathy will be grouped according to patient's body mass index. Atrial trabeculae will be explored to dissect out the respective contribution of (i) sarcoplasmic reticulum dysfunction (isometric twitch force contraction analysis), (ii) abnormal sarcomere sensitivity to calcium assessed in permeabilized fibers and (iii) mitochondrial dysfunction to intrinsec contractile dysfunction of myocardium in obese patients.

Study Timeline

• Study Start: 2013-06-03
• Study First Submitted: 2015-03-04
• Study First Submitted QC: 2015-03-09
• Study First Posted: 2015-03-10
• Primary Completion: 2017-01-19
• Study Completion: 2017-01-19
• Status Verified: 2019-07
• Last Update Submitted: 2019-07-09
• Last Update Posted: 2019-07-10

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 134

Inclusion Criteria

• patient schedulled for coronary artery bypass graft surgery or aortic valve replacement surgery with extracorporeal circulation
• must be over 18 years of age
• must be able to read and understand the consent form in French
• must be in sinus rhythm at inclusion time

Exclusion Criteria

• medical history of type 1 diabetes
• medical history of sustained atrial arrhythmia
• pregnancy
• class III anti-arhythmic drug

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
obese patients	functional tissu atrial analysis	patients with BMI \> 30
no obese patients	functional tissu atrial analysis	norma weight patient (BMI\<25) overweight patients ( 25\< BMI \>30)

Primary Outcome Measures

Name	Time	Method
Intrinsic myocardial contractile force	the day of cardiac surgery	twitch force developed by atrial trabeculae assessed in isometric conditions in atrial samples

Secondary Outcome Measures

Name	Time	Method
sarcomere sensitivity to calcium	the day of cardiac surgery	force-calcium concentration relation assessed on permeabilized atrial fibers
Mitochondrial function	the day of cardiac surgery	assesment of mitochondrial respiration and calcium retention capacity by oxygraphic measurement in saponin-permeabilized atrial fibers

Trial Locations

Locations (1)

Hôpital Cardiologique - EFCV - CHRU; 🇫🇷 Lille, France