Hydrogen Peroxide Fumigation in Dental Office Environment
- Conditions
- Bacterial InfectionsAerosol DiseaseDental Caries
- Registration Number
- NCT06100848
- Lead Sponsor
- Wroclaw Medical University
- Brief Summary
The study's null hypothesis posits no significant difference in bacterial levels in the dental office environment before and after implementing hydrogen peroxide (H₂O₂) fumigation. The study comprised 30 participants, 18 females and 12 males, all diagnosed with moderate caries decay (ICDAS 3 and 4) in their mandibular molars, averaging 42.2 ± 8.3 years in age. Sample size calculations for 30 microbiological plates in each group utilized G\*Power software (Kiel University, Germany), factoring in prior research, with a significance level of 0.05, effect size (d) of 0.72, 95% confidence interval, and 85% power. Aerobic bacterial content in the dental office air was assessed using the Koch sedimentation method. The study employed 60 Petri dishes with Columbia Agar and 5% Sheep Blood. During caries treatment, thirty plates were opened and sealed 40 minutes later, while another set of thirty plates was opened and closed 60 minutes post-fumigation. Measurements were taken 1 meter above the ground and 2 meters from the patient's mouth. After 48 hours of incubation at 37°C, microbiological contamination was calculated as CFUs (colony-forming units) in one cubic meter using the formula: L = a × 1000 / (πr² × k). Fumigation involved a 20-minute treatment with 6% hydrogen peroxide biosanitizer (Saniswiss, Switzerland) via a compressed air device (Fumi-Jet, Kormed, Poland). The process included 3 minutes of fumigation and a 17-minute waiting period for the chemotoxic effect, with 45 ml of 6% hydrogen peroxide sprayed in a 20 m² room.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 30
- diagnosed with moderate caries decay based on the International Caries Detection and Assessment System (ICDAS 3 and 4) in their mandibular molar teeth
- received hygienist treatment two weeks before the study initiation
- use anti-inflammatory medications
- non-smokers
- had systemic illnesses
- with uncompensated diabetes
- with halitosis symptoms
- with gastric diseases
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Primary Outcome Measures
Name Time Method Number of bacteria after caries treatment After 48 hours of incubation At the onset of caries treatment, thirty plates (n=30) will be opened and will be sealed 40 minutes later.
After 48 hours of incubation at 37°C, the degree of microbiological contamination will be determined, calculated as the total number of CFUs (colony-forming units) in one cubic meter of air using the formula: L = a × 1000 / (πr² × k). In the formula, L represents the microbial contamination level in \[cfu/m3\], 'a' signifies the quantity of bacterial colonies cultivated on the plate, 'r' denotes the Petri dish radius \[cm\], and 'k' stands for the plate exposure time factor, with k = t × 1/5, where 't' represents the exposure time in minutes.Number of bacteria after caries treatment and fumigation After 48 hours of incubation Another set of thirty plates (n=30) will be opened immediately before caries treatment and closed 60 minutes later following the completion of fumigation. After 48 hours of incubation at 37°C, the degree of microbiological contamination will be determined, calculated as the total number of CFUs (colony-forming units) in one cubic meter of air using the formula: L = a × 1000 / (πr² × k). In the formula, L represents the microbial contamination level in \[cfu/m3\], 'a' signifies the quantity of bacterial colonies cultivated on the plate, 'r' denotes the Petri dish radius \[cm\], and 'k' stands for the plate exposure time factor, with k = t × 1/5, where 't' represents the exposure time in minutes.
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
Oral Surgery Department
🇵🇱Wroclaw, Poland
Oral Surgery Department🇵🇱Wroclaw, Poland