Alterations in Subgingival Microbiota and Hypoxia in Occlusal Trauma

Completed

• Conditions: Chronic Periodontitis; Occlusal Trauma

• Registration Number: NCT03621891
• Lead Sponsor: Tokat Gaziosmanpasa University

Brief Summary

Present study suggests that excessive occlusal forces did not cause a significant change in hypoxia and vascular markers, however a slight alteration in subgingival microbiota was observed.

Detailed Description

Occlusal trauma is defined as tissue destruction and/or injury resulting in tissue changes in the periodontium as a consequence of excessive occlusal forces or hyper-occlusion. Previous studies suggested accelerated periodontal destruction around teeth with occlusal trauma and increased mobility, however, the evidence is lacking. Recent animal studies found that occlusal trauma increased osteoclastic activity and alveolar bone loss. One possible mechanism suggested for the progression of periodontal destruction with occlusal trauma is that tooth mobility creates a subgingival environment conducive to overgrowth by periodontal pathogens. However, the association of occlusal trauma and subgingival microbiota was not evaluated in previous studies. Therefore, present study aimed to evaluate subgingival dental plaque components and compare with healthy individuals and periodontitis patients without occlusal trauma.

Study Timeline

• Study Start: 2016-05-01
• Primary Completion: 2017-05-01
• Study Completion: 2017-05-01
• Status Verified: 2018-08
• Study First Submitted: 2018-08-04
• Study First Submitted QC: 2018-08-04
• Last Update Submitted: 2018-08-08
• Study First Posted: 2018-08-09
• Last Update Posted: 2018-08-10

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 80

Inclusion Criteria

• Systemical health
• No drug use
• No pregnancy or lactation For group 3 and 4;
• Bruxism at least for 2 years
• Occlusal trauma
• Existence of at least 20 functioning teeth

Exclusion Criteria

• Smoking
• Pregnancy/lactation
• Systemical disease
• Drug use
• Absence of at least 20 functioning teeth

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Determination of certain bacterial species in subgingival dental plaque	A day after initial examination	Subgingival dental plaque samples were collected from each participant and evaluated via polymerase-chain reaction.

Secondary Outcome Measures

Name	Time	Method
hypoxic changes in gingiva and serum	One day after initial examination	Determination of the hypoxia inducible factor-alpha and vascular endothelial growth factor via ELISA

Trial Locations

Locations (2)

Gaziosmanpasa University Faculty of Dentistry; 🇹🇷 Tokat, Turkey

Gaziosmanpasa University; 🇹🇷 Tokat, Turkey